作　　者： ; ; ; ; ; ; ; ; ; ;

机构地区： 深圳出入境检验检疫局

出　　处： 《中国预防兽医学报》 2008年第5期375-378,共4页

摘　　要： 为了建立检测口蹄疫的快速实用分子生物学技术,本研究借助新兴的环介导等温扩增技术,建立了一种灵敏、特异、快速的分子生物学检测方法-体外等温扩增检测方法。设计了6条针对FMDV3D基因的8个位点的特异性引物,建立了一套从核酸抽提到检测仅需要75min的快速检测技术。所建立的检测方法灵敏,与实时荧光RT-PCR灵敏度相当;且具有特异性,对其他水泡性疾病病原体检测均为阴性;而且简单,不需要复杂的仪器,常规水浴锅在63.5℃即可进行,肉眼可直接观察检测结果。该方法是适合基层和现场检测而无需送至中心实验室的快速灵敏实用的分子生物学检测方法。 In order to develop a rapid and simple technology for diagnosis of foot-and-mouth disease vires （FMDV）, a novel speed RT-LAMP assay for FMDV was explored with Loop-mediated amplification （LAMP）. 3 pairs of primers to identify 8 positions in 3D gene were designed. A RT-LAMP assay was developed for the rapid detection of FMDV. The process of assay was conducted with one step amplification at 63.5 ℃ in a single tube within 75 minutes and the amplification results was visualized by adding SYBR. The sensitivity of RT-LAMP assay was equivalent to the real time RT-PCR and no amplification was found with the samples of SVDV, PRRSV, SVDV. Therefore, the rapid and simple assay is a potential useful technique for FMDV detection in field conditions.

关 键 词： 口蹄疫病毒 环介导等温扩增技术

领　　域： [生物学] [农业科学] [农业科学] [农业科学]