机构地区: 华南农业大学园艺学院
出 处: 《安徽农业科学》 2008年第9期3538-3539,共2页
摘 要: [目的]快速筛选出番茄青枯病抗性基因的SSR标记。[方法]对来自4个国家27份番茄材料青枯病的抗性进行鉴定,并用一套SSR标记对材料进行分析,寻找与青枯病抗性基因连锁的标记。[结果]来自国内的3份材料表现出理想的抗病性,"湘引79-1"表现高抗,"860"及"039-3"表现抗病。标记TOM176-177在3份抗病材料上能扩增出相同带型,而在其他材料上扩增出的带型不同。TOM176-177在27份材料上存在4个等位基因,在3份抗病材料上是同一个等位基因,其扩增产物比其他等位基因小。[结论]该研究初步推断标记TOM176-177与青枯病抗性基因连锁。 Twenty-seven tomato materials collected from four countries were evaluated in bacterial wilt resistance with artificial inoculation and PCR analysis were performed with SSR markers to identify the potential marker linked to bacterial wilt resistance. The results showed that "Xiangyin79- 1"was highly resistant,"860" and "(139-3"were resistant to this disease,An SSR marker Tom176-177 had a band pattern different from all other materials with less resistance,It was inferred that this marker was linked with bacterial wilt resistance.