作　　者： ; ; ; ; ; ;

机构地区： 南方医科大学南方医院

出　　处： 《南方医科大学学报》 2008年第4期658-661,共4页

摘　　要： 目的研究重组人血管内皮抑素在体外对人肝癌HCCLM6细胞粘附、迁移及侵袭的抑制作用。方法分别以25、50、100、200、400μg/ml的重组人血管内皮抑素在体外处理HCCLM6细胞,采用MTT法检测药物对细胞增殖的影响,利用transwell小室及matrigel胶体外检测药物对细胞迁移、侵袭及粘附的影响。结果重组人血管内皮抑素作用HCCLM6细胞72h后对细胞增殖存在一定的抑制作用;重组人血管内皮抑素可明显抑制HCCLM6细胞与matrigel的黏附、对人工基底膜的侵袭力和迁移力,且抑制作用均呈剂量依赖性。结论重组人血管内皮抑素具有直接抑制人肝癌细胞体外侵袭转移和增殖作用,其机制有待进一步探讨。 Objective To investigate the inhibitory effects of recombinant human endostatin （rh-ES） on cell adhesion, metastatic potential and invasiveness of hepatocellular carcinoma HCCLM6 cells in vitro. Methods The changes in the cell proliferation status of HCCLM6 cells treated with different concentrations of rh-Endostatin in vitro was measured with MTT assay, and their invasiveness and migration were assayed using transwell cell culture chamber. The cell adhesion assay was carried out on 96-well plate precoated with matrigel. Results Rh-ES showed inhibitory effect against the proliferation of HCCLM6 cells atter a 72-h treatment. The adhesion, metastatic potential and invasiveness of the cells were obviously inhibited by rh-Endostatin in a dose-dependent manner. Conclusion Rh-ES inhibits the adhesion, invasiveness and migration ofhepatocellular carcinoma cells in vitro, and the mechanism needs further investigation.

关 键 词： 重组人血管内皮抑素 肝癌 细胞 转移 侵袭

领　　域： [生物学]