作　　者： ; ; ; ; ;

机构地区： 华南理工大学生物科学与工程学院

出　　处： 《生物学杂志》 2008年第2期26-29,共4页

摘　　要： 为探讨鲎素体外分子结构稳定性，通过温度、pH值、体外蛋白酶分别处理鲎素，采用HPLC法检测鲎素残留量及评定鲎素分子结构稳定性情况。结果表明：在t〈120℃或120℃作用10min以内，pH值〈10．0以及胃置白酶作用情况下，鲎素的质量未发生明显变化，分子结构稳定，高效液相色谱检测鲎素残留量在65．5～70．2μg之间，与未处理对照样相差不大；当在120℃处理30min以上，pH值〉10．5，或则在胰蛋白酶、弹性蛋白酶、羧肽酶作用下鲎素质量明显下降，鲎素分子结构被破坏，鲎素残留为痕量。 In order to study the molecular structure＇s stability of tachyplesin, the tachyplesins were processed by a series of different temperature,pH and proteinase in vitro. The residual content of tachyplesin was measured by the high performance liquid chromatography. The molecular structure＇s stability was analyzed. The results indicated that, when the temperature was under 120℃, the initial pH value under pHI0. 0, the tachyplesin was conlpletely stable in the molecular structure, the residual content of tachyplesin was from 65.5 μg to 70.2μg,tachyplesin was kept stable in pepsin stock concentration solution ？ Howerver, when the temperature was above 120℃, the initial pH value above pH10.0, the quality of tachyplesin decreased obviously, tachyplesin was hydrolyzed in stock concentration solution of trypsin, elastase and carboxypeptidase B.

关 键 词： 高效液相色谱 鲎素 值 温度 蛋白酶

领　　域： [生物学]