作　　者： ; ; ; ; ;

机构地区： 广东教育学院

出　　处： 《中山大学学报（自然科学版）》 2008年第2期99-102,共4页

摘　　要： 以成功克隆大蕉几丁质酶基因(MpChi-1)时设计的一对引物,采用RT-PCR技术从广亲和水稻品种(Oryza.sativalL.Cpslo17)中扩增到一水稻几丁质酶的全长cDNA,长1 115 bp,命名为Oschi;此序列已在GenBank中注册,登录号为EU045451;将Oschi基因核苷酸测序结果在NCBI服务器上用BLAST搜索软件进行同源性基因检索;并将Oschi的cDNA序列及氨基酸序列与大蕉及其它单子叶植物的相应序列进行多重序列排列比较并构建系统树;结果显示此基因具有编码Ⅰ类几丁质酶的基本结构;与大蕉核苷酸序列相比同源性为99%,氨基酸序列同源性为99%,与预测登记的最相近的水稻[Oryza sativa(japonica cultivar-group)]核苷酸序列相比同源性为98%,氨基酸序列同源性为98%;广亲和水稻Oschi基因与大蕉MpChi-1基因的序列及蛋白结构有较高的一致性。 Using a degenerate primer designed for cloning chitinase cDNA from plantain banana （MpChi - 1 ）, we obtained a full-length cDNA clone named Oschi from rice by RT - PCR. The rice chitinase cDNA was 1 115 bp long and registered in GenBank with the accession number, EU045451. Using Clustal W online, we compared the cDNA sequence and amino acid sequence of Oschi with that from the plantain banana and the other monocotyledon, and constructed a phylogeny tree. The result shows that Oschi has some basic structures which are common among chitinase I gene. It shares a 99% homology with the gene sequence as well as amino sequence with those from plantain banana, and a 98% homology with that from Oryza sativa （japonica cultivar-group） which has been reported. These evidences supported that Oschi and Mpchi - 1 were highly identical to the gene sequences and protein structures.

关 键 词： 几丁质酶 基因 克隆 序列分析 水稻 遗传工程

领　　域： [生物学]