作　　者： ; ; ;

机构地区： 暨南大学生命科学技术学院化学系

出　　处： 《海洋通报》 2008年第2期52-58,共7页

摘　　要： 在钝顶螺旋藻(S.platensis)生长期内(1～11d)添加无机碲(Na2TeO3),每天的碲剂量水平χTe分别为0.05,0.1,0.2,0.3,0.4,观察S.platensis对不同剂量水平无机碲的吸收转化作用,同时考察χTe为0.4的实验组螺旋藻中碲与生物大分子(蛋白质、多糖、核酸和脂类)的结合情况。结果表明,当χTe<0.3时,碲对S.platensis的生长有促进作用;当χTe>0.3时,S.platensis的生长受到明显的抑制。无机碲进入藻体后一部分被转化成有机碲并进入生物分子中,而绝大部分却以气态碲(甲基化碲)的形式排出,且占添加碲的86.7%～91.6%,气态碲的产生可能是螺旋藻对碲的主要解毒方式。气态碲的含量随着碲剂量的增加而增加,而有机碲的含量随着碲剂量的增加而减少。碲与S.platensis中的3种组分(组分1:固体残余物,组分2:水溶性蛋白,组分3:非蛋白质水溶性物质)有不同程度的结合,不同组分中的碲相对含量随着碲剂量的变化有明显的变化:当χTe≤0.1时,组分2和组分3的碲含量较大;当χTe≥0.2时,组分1和组分2中的碲含量较大。进入生物大分子的碲主要与蛋白质相结合。 Tellurite was added to S. platensis in its growing period （ 1 - 11 d ）, and there were five test sets that ХTe were 0.05, 0.1, 0.2, 0.3, 0.4 respectively everyday. The effects of different ХTe on the growth of S. platensis and the biotransformation of Te were studied. The combination between Te and the biological molecules including protein, polysaccharides, lipids and nucleic acid was researched. It indicated that the growth of S. platensis was promoted when ХTe 〈 0.3. When ХTe 〉 0.3, and its growth was inhibited. Tellurite was transformed into organic tellurium and combined with the biological molecules. Most of tellurite was discharged from cells by the way of gaseous Te that might be the way to detoxification of S. platensis no Te, and it was 86.7 % - 91.0 % of the Te added. The content of gaseous Te increased with ХTe＇S increasing, while the content of organic Te decreased with ХTe＇S increasing. Te was combined to three different components. And the contents of Te in three components have obvious changes： when ХTe≤ 0.1, its contents in component 2 and 3 were higher, while its content in component 1 and 2 were higher at ХTe≥ 0.2. Te was combined to the biological molecules, most of which were combined to protein. The content of Te in protein was the highest.

关 键 词： 碲剂量 钝顶螺旋藻 生物转化 生物分子 气态碲

领　　域： [生物学]