作　　者： ; ; ; ;

机构地区： 西北农林科技大学动物科技学院

出　　处： 《西北农林科技大学学报（自然科学版）》 2008年第3期80-84,共5页

摘　　要： 【目的】探索高效苦马豆素(SW)人工抗原SW-BSA的合成方法。【方法】设计新的技术路线,合成SW人工抗原制备的关键中间产物SW-对甲基苯甲酸,并进行波谱分析。应用EDC法将SW-对甲基苯甲酸分别与牛血清白蛋白(BSA)和卵清蛋白(OVA)连接,制备免疫抗原SW-BSA和包被抗原SW-OVA,通过小鼠免疫试验检测所合成的SW-BSA的免疫原性。【结果】合成的SW-对甲基苯甲酸的结构与预期目标产物一致;合成的SW-BSA可刺激小鼠产生针对SW的抗体;通过薄层层析技术可对反应进程进行即时检测。【结论】SW-BSA人工抗原合成成功,该人工抗原具有良好的免疫原性。 【Objective】 The study was to search for an efficient SW-BSA method to synthesize the SW artificial antigen.【Mehtod】 A new synthetic route was used to synthesize the key intermediate product,SW-4-methylbenzonic acid,then it was coupled with BSA or OVA by EDC·HCl to produce SW-BSA and SW-OVA,the immunogenicity of the SW-BSA was determined by inoculating the mice.【Result】 The key intermediate product,SW-4-methylbenzonic acid,was synthesized successfully.The spectral analysis of structure verified that it was absolutely coincident with that deduced theoretically with the right molecular weight.The immunologic tests showed that the serum antibody against SW in inoculated mice were high,indicating the satisfactory immunogenicity of the SW artificial antigen.【Conclusion】 The results indicated that new synthesis route was practical and efficient for synthesis of SW-BSA,which had a favorable immunogenicity.

关 键 词： 苦马豆素 人工抗原 免疫原性

领　　域： [农业科学] [农业科学] [农业科学]