作　　者： ; ; ; ; ; ; ; ;

机构地区： 南方医科大学公共卫生与热带医学学院流行病学系

出　　处： 《现代预防医学》 2008年第5期870-872,878,共4页

摘　　要： [目的]为获得生菜高频率的离体再生体系。[方法]采用正交试验设计,探讨不同生菜品种、不同消毒时间对培养生菜无菌苗的影响,并研究不同浓度的植物生长调节剂、AgNO3和不同外植体切段对生菜外植体芽分化及生根成苗的影响。[结果]美国大速生品种用70%乙醇浸泡60s,再用2%次氯酸钠处理15min,发芽率达到80%,无菌苗生长好;生菜离体培养再生芽的优化分化条件组合为MS(Murashige and Skoog’s)培养基附加BA(6-苄基腺嘌呤,6-benzylaminopurine)0.5mg/L,IAA(吲哚乙酸,indole-3-acetic acid)0.5mg/L,外植体为2～3d的子叶;生菜再生芽在含低浓度的生长素NAA(萘乙酸,α-naphthaleneacetic acid)浓度为0.05mg/L,或IAA浓度为0.5mg/L的1/2MS培养基中生根情况较好。[结论]优化的生菜离体再生体系具有很好的重复性,可用于遗传转化获得保健生菜。 [ Objective ] In order to get high-frequency regeneration system in vitro culture of lettuce. [ Methods] Used the orthogonal experiment design to explore the effects of different breed and sterilization time on the sterile seedling of lettuce and to study the effects of different plant growth regulators, AgNO3 and explants on induction of adventitious buds and advenfitious roots. [ Results ] The germination rate reached 80% and the sterile seedlings grew well when the lettuce seeds were treated for 60 seconds with 70% ethanol, and then were sterilized with 2% sodium hypochlorite for 15 minutes. The optimized condition of regeneration system in vitro culture of lettuce was MS medium supplementing with 0.5 mg/L BA, 0.5 mg/L IAA, then used the cotyledon of seedling of 2-3 days as explants. The adventitious roots grew well in 1/2MS medium containing 0.05 mg/LNAA or 0.5 mg/L IAA. [Conclusion] The optimal regeneration system in vitro culture of lettuce could be repeated well, and could be used for genetic transformation

关 键 词： 生菜 外植体 离体培养 植物生长调节剂 植株再生

领　　域： [农业科学] [农业科学]