作　　者： ; ; ; ; ;

机构地区： 西南大学生命科学学院

出　　处： 《北方园艺》 2008年第2期193-197,共5页

摘　　要： 采用生物信息学方法和工具对GenBank中的洋葱、豌豆、番茄和茶等植物的黄酮类化合物合成关键酶查尔酮异构酶(CHI)的核酸和氨基酸序列进行了比对、分析和建模,进而对其分子结构、理化性质、亚细胞定位、蛋白转运肽、跨膜结构域、疏水性、分子系统进化、蛋白质二级和三级结构等重要参数进行了预测和推理。结果表明:该类酶基因的全长包括5′、3′非翻译区和一个开放阅读框,无蛋白转运肽,且定位于细胞质基质,是一个疏水性蛋白,二级结构均以随机卷曲和α-螺旋为主要构件,洋葱和豌豆的CHI三维建模成功。 The nucleic acid sequences and amino acid sequences of chalcone isomerase from Alliurn cepa, Pisum sativurn, Lycopersicon esculentum and Camellia sinensis, which were registered in GenBank, were analyzed and predicted by the tools of bioinformatics in the following aspects： the composition of nucleic acid sequences and amino acid sequences, molecular structure, physical and chemical characters, subcellular localization, transitpeptides, transmembrane topologieal structure, hydrophobicity, molecular phylogenetic evolution, secondary and tertiary structure of protein and so on. The results as following： the full-length gene of CHI contains an opening reading frame, 5＇-untranstrated region and 3＇-untranstrated region, all lie in cytoplasm, and haven＇t transmembrane topologieal structure,were a hydrophic protein α-helix and random coil were main component all secondary structures. The 3D models of Allium cepa and Pisum sativum areconstructed.

关 键 词： 查尔酮异构酶 生物信息学 黄酮类化合物

领　　域： [生物学] [生物学]