作　　者： ; ; ; ; ; ; ; ;

机构地区： 军事医学科学院野战输血研究所

出　　处： 《制冷学报》 2008年第1期28-32,共5页

摘　　要： 对小鼠卵巢组织进行玻璃化深低温保存的初步研究,探索合适的玻璃化保护剂及保存方法。取3周龄小鼠卵巢组织,采用不同保护剂进行玻璃化深低温保存,-196℃液氮保存一周后复温,进行组织切片HE染色观察,筛选合适的玻璃化保护剂;对保存后的小鼠卵巢组织进行自体移植体内培养2周后,取出卵巢组织进行形态学观察及基因表达研究,采用RT-PCR方法检测到了在原始卵泡向初级卵泡发育过程中特异表达的AMH基因及在各个阶段的卵泡中均表达的KL基因。结果表明采用高浓度渗透性保护剂(二甲基亚砜、乙酰胺、丙二醇)的玻璃化保护液对小鼠卵巢组织保存效果较好,说明采用玻璃化技术保存卵巢组织的方法可行,并可采用检测基因表达的方法验证深低温保存卵巢组织的效果。 The research on cryopreservation of mouse ovary tissues was performed to explore suitable vitrification solutions and preservation methods. Ovary tissues of 3-week old mouse were dissected and cut into pieces, and then they were cryopreserved with different vitrification solutions. The tissues were kept at -196℃ for one week and then reheated. The best vitrification solution was selected according to the histological observation on the tissue slices. The reheated ovary tissues were autotransplanted into the kidney capsules. Two weeks later, the autotransplanted tissues were taken out for evaluation. RT-PCR was used to detect AMH gene and KL gene. The results show that vitrification solution composed of infiltrative agents with high concentration could yield excellent effect on preservation of mouse ovary tissues. Our conclusion is that vitrification of mouse ovary may be feasible and detection of gene expression could be applied to validate the quality of cryopreservation.

关 键 词： 细胞生物学 小鼠卵巢 玻璃化 基因 基因

领　　域： [生物学] [一般工业技术]