作　　者： ; ; ; ; ; ; ;

机构地区： 湖南大学环境科学与工程学院

出　　处： 《环境科学学报》 2008年第2期319-325,共7页

摘　　要： 筛选出一株能分泌胞外酶、促进污泥溶解的嗜热脂肪芽孢杆菌Bacillus stearothermophilus sp.AT06-2,将AT06-2培养液接种入不同浓度(TSS约为7、14和21g·L-1)的剩余污泥,于60℃进行嗜热菌溶解试验,并与未接种试验进行对比.结果表明,接种后TSS和VSS溶解率都得到提高.60h后接种试验VSS溶解率分别达到58.76%、50.17%和39.38%,而未接种试验VSS溶解率仅为34.00%、28.63%和25.22%.在固形物质溶解机理方面,VSS的溶解主要受酶催化效应和热水解效应的影响,TSS的溶解减量取决于VSS和FSS的组成比例.VSS溶解在初始阶段(t=3d)符合准一级反应动力学模型,接种后溶解速率常数Kd显著提高,分别从未接种的0.14d-1、0.10d-1、0.10d-1相应提高到接种条件下的0.30d-1、0.23d-1和0.16d-1. A thermophilic bacterium AT06-2 identified as Bacillus stearothermophilus sp. was isolated and shown to produce extraeellular enzymes accelerating to solubilize excess sludge. Sludge solubilization with and without inoculation of AT06-2 was investigated at 60℃ under different total suspended solid （TSS） concentrations （7, 14 and 21 g·L^-1）. The results showed that the solubilization of TSS and volatile suspended solids （VSS） were enhanced by inoculation with the bacteria. The VSS solubilization rate reached 58.76% , 50. 17% and 39.38% 60 hours after the inoculation, compared to 34.00% , 28.63% and 25.22% of VSS solubilized without inoculation under the same conditions. VSS solubilization was mainly affected by enzyme-catalysis and thermal hydrolysis, and TSS minimization was largely determined by the component ratio of VSS and fixed suspended solid （FSS）. Pseudo-first order reaction kinetics could adequately describe VSS solubilization for the first 3 days and the soluhilization rate constant K_d （t=3d） inereased greatly from0.14 d^-1, 0.10d^-1 and0.10 d^-1 to 0.30 d^-1, 0.23d^-1and0.16d^-1 with AT06-2 inoculation.

关 键 词： 剩余污泥 溶解 嗜热菌 胞外酶 准一级反应动力学

领　　域： [环境科学与工程]