作　　者： ; ; ;

机构地区： 宁波大学材料科学与化学工程学院宁波市新型功能材料及其制备科学国家重点实验室培育基地

出　　处： 《应用化学》 2008年第2期189-193,共5页

摘　　要： 将四羧基钌(Ⅱ)酞菁(RuPc)共价键合到自组装在Au电极表面的巯基丁二胺单分子层上,获得了自组装单分子膜修饰金电极(RuPc-CME),并对其进行了IR、XPS表征。该修饰电极对L-半胱胺酸(Hcy)的氧化显示出较好的电催化响应,可用作Hcy的电化学传感器。探讨了传感器对Hcy的电催化氧化机理pH=5.5,考察了底液pH值的影响。传感器的计时电流曲线的响应时间小于5s,氧化电流与Hcy浓度在5.0×10-7～5.4×10-5mol/L范围内成良好线性关系,检测下限为1.8×10-7mol/L(S/N=3),可用于检测人血清样品中的Hcy。 One monolayer containing thiol-tailed groups was formed via the covalent binding of Ru（ Ⅱ ） phthlocyanine（RuPc） on a dimercaptosuccinic amine-modified gold electrode by self-assembly, which was characterized by IR and XPS. The modified gold electrode showed an excellent electrocatalytic activity for the oxidation of homocysteine（Hcy） in a phosphate buffer solution （pH = 5.5 ）. The electrocatalytic mechanism and effect of pH of the buffer solution were also investigated. The steady-state current of chronoamperometry increased linearly with Hcy concentration in the range of 5.0 × 10^-7 ～ 5.4 × 10^-5 mol/L with a detection limit of 0. 18μmol/L（ S/N = 3 ）. The sensor also showed excellent reproducibility that makes it an ideal amperometric detector for Hcy in human serum.

关 键 词： 金电极 自组装单分子层 电催化 钌 酞菁 半胱胺酸

领　　域： [理学] [理学]