作　　者： ; ; ; ; ;

机构地区： 中国水产科学研究院珠江水产研究所

出　　处： 《上海水产大学学报》 2008年第1期22-27,共6页

摘　　要： 应用RAPD技术对3个奥利亚罗非鱼(Oreochromis aureus)群体(奥利亚罗非鱼83系、新引进的红色奥利亚罗非鱼与奥利亚罗非鱼02系)进行遗传多样性分析。根据扩增结果统计获得群体内和群体间的遗传距离:奥利亚罗非鱼83系、红色奥利亚罗非鱼、奥利亚罗非鱼02系群体内的遗传距离分别为0.021 0,0.090 3和0.014 9;群体间的遗传距离以奥利亚罗非鱼83系与红色奥利亚罗非鱼群体间的最大,为0.135 1,奥利亚罗非鱼83系与奥利亚罗非鱼02系的遗传距离最小,为0.061 0,红色奥利亚罗非鱼与奥利亚罗非鱼02系群体间的遗传距离为0.121 8。利用MEGA3.1软件进行聚类分析,奥利亚罗非鱼83系与奥利亚罗非鱼02系首先聚为一支,然后再与红色奥利亚罗非鱼聚为一支。3个奥利亚罗非鱼群体总的Shannon多样性指数为:0.234 0,总的Nei基因多样性指数为0.152 7,3个群体的遗传分化指数(Gst)为0.507 1。上述结果说明这3个群体的群体内遗传变异较小,但群体间存在一定程度的遗传变异和遗传分化。对RAPD分析中所获得的6条特异带进行SCAR转化。S97800和S4691400两个特异片段成功转化为SCAR标记。这些标记可作为鉴别3个奥利亚罗非鱼群体的遗传标记,并将为今后的选择育种提供分子标记。 Using RAPD technique, the genetic diversity of 3 populations of Oreochromis aureus （ O. aureus, red O. aureus and blue O. aureus ） was analyzed. The genetic distances of intrapopulation for O. aureus, red O. aureus and blue O. aureus were 0.021,0. 090, and 0. 014 9. The genetic distances of interpopulation were 0. 135 1,0. 060 7 and 0. 121 8. The maximum occurred between red O. aureus and O. aureus, the minimum did between O. aureus and blue O. aureus. Clustering analysis with the methods of UPGMA and NJ in MEGA3.1 on the basis of genetic distances, the results showed the populations of O. aureus and blue O. aureus assembled one branch first, then did red O. aureus. The results also showed that the 3 populations of O. aureus had a total Nei＇s value of 0. 152 7 for gene diversity and the Shannon＇s information index of 0. 234 0. The gene differentiation coefficient（Gst） is 0. 507 1. There was a relatively low genetic diversity intrapopulation, but there was genetic differentiation of a certain degree among the 3 populations. On 6 population-specific RAPD bands SCAR transformation was performed. The results showed that the two specific bands S97800 and S4691400 were transformed into SCAR markers successfully. They could be used as specific marker for population identification, and will likely benefit future selective breeding programs as reliable and reproducible molecular markers.

关 键 词： 奥利亚罗非鱼 遗传多样性 随机扩增多态 标记

领　　域： [农业科学]