作　　者： ; ; ;

机构地区： 广州军区广州总医院

出　　处： 《免疫学杂志》 2008年第1期97-99,共3页

摘　　要： 目的制得乙肝免疫传感器,用于临床检验。方法以金电极为基底,自组装半胱胺,通过纳米金静电竞争吸附硫堇分子和乙肝过氧化酶酶标抗体,制得乙肝免疫传感器。采用循环伏安法和线形扫描伏安法考察了传感器的组装过程,响应电流的性质,传感器对乙肝病毒的响应特性。结果对乙肝抗原进行了定量分析,线形范围为1∶30～1∶200(稀释浓度V);线性相关系数R为0.994。取临床血清进行检测,将结果与临床常用的ELISA法比较,符合要求。结论用半胱胺/纳米金方法固定乙肝抗体制得免疫传感器能应用于临床检验。 Objective To construct a novel electrochemical immunosensor for detection of HBV antibody. Methods Cystamine was assembled on gold electrode to form a self-assemble monolayer. Then the amino-groups could connect with the gold nanoparticles in Au colloid solution by electrostatic binding. After the gold nanopartieles adsorbed, the thionine and the HBV horseradish peroxidase （HRP） antibody were electrostaficaUy botmded to the self-assemble monolayer competitively to form the Hepatitis B electrochemical immtmosensor. Cyclic voltammetry and linear scan voltammetry were used to investigate the assembling process and the chemical characteristics of the immunosensor. Results The linearity of HBV was in the range of 1：30 - 1：200 with the correlation coefficient of 0.994. While applying in human serum detection, this method was in good agreement with the enzyme linked immunosorbent assay. Conclusion The HBV electrochemical immunosensor formed by this method can be used in the clinical detection of HBV.

关 键 词： 自组装 乙肝 半胱胺 纳米金 免疫传感器

领　　域： [自动化与计算机技术] [自动化与计算机技术]