作　　者： ; ; ; ;

机构地区： 江苏农牧科技职业学院

出　　处： 《辽宁化工》 2007年第12期824-826,共3页

摘　　要： 目的建立反相液相色谱分离二极管阵列双波长检测法测定复方泰妙林注射液中泰妙菌素、磺胺嘧啶钠的含量的方法。方法采用Shim-packCLC-ODS柱（150mm×6mm，5μm），流动相为80％乙腈-0．7％磷酸氢二铵（体积比为0．3：0．7），并用氨试液调节pH至8．0±0．1，流速为1．0mL／min，泰妙菌素和磺胺嘧啶钠的检测波长分别为208nm、241nm，柱温为40℃。结果在此色谱条件下两者能完全分离，泰妙菌素和磺胺嘧啶钠分别在5-40μg／mL、10-80μg／mL范围内，浓度与峰面积呈良好的线性关系（R=0．9999），泰妙菌素和磺胺嘧啶钠回收率分别为99．60％-99．99％和99．70％-100．3％，相应的相对标准偏差分别为0．40％～0．75％和0．45％～0．80％。结论方法简便、快速、准确，可用于复方泰妙林注射液的含量测定。 Objective： To establish a PDA - RP - HPLC method for determination of tiamulin and sulfadiazine sodium in compound tiamulin injection. Shim - pack CLC - ODS column （ 150 mm × 6 mm, 5 μm ） was used. The mixture of 80 % acetiontrile - 0.7 % ammonium dihydrogen phosphate （0.3：0.7 ） was adopted as the mobile, and adjusted to pH 8.0 ± 0.1 by ammonia liquor. The flow rate was 1.0 mL/min. The wavelengths 208rim and 241rim were used for detection of tiamulin and sulfadiazine sodium, respectively. The temperature of column was 40 ℃. Results： Under the adopted chromatographic condition, the tiamulin and sulfadiazine sodium could be completely separated. The standard curves for tiamulin and sulfadiazine sodium were linear in the concentration range of 5 - 40 μg/mL and 10 - 80 μg/mL （ R = 0.9999）, respectively. The average recoveries of tiamulinand and Sulfadiazine Sodium were 99.60 % - 99.99 % and 99.70 % - 100.3 %, with RSD 0.40 % - 0.75 % and 0. 45 % - 0.80 %, respectively. Conclusions ： The method was convenient, rapid, accurate for determination of tiamulin and sulfadiazine sodium in compound tiamulin injection.

关 键 词： 反相液相色谱法 复方泰妙林注射液 泰妙菌素 磺胺嘧啶钠

领　　域： [理学] [理学]