作　　者： ; ; ; ;

机构地区： 中国农业大学食品科学与营养工程学院

出　　处： 《食品与发酵工业》 2007年第11期103-106,共4页

摘　　要： 以美国、阿根廷、巴西转基因大豆等为材料,建立了ELISA法定量检测抗草甘膦转基因大豆CP4 EP- SPS蛋白的方法,成功检测出美国转基因大豆含量为3.768%、阿根廷转基因大豆含量为2.820%、巴西转基因大含量为1.920%,转基因豆粉、转基因豆粕和中国大豆未检测到CP4 EPSPS蛋白。此方法具有简便、快速、稳定等优点,其检测灵敏度可达到0.0075%,并且稳定性良好,为抗草甘膦转基因大豆中CP4 EPSPS蛋白的定量检测提供了有效的手段。 Using the genetically modified soybean from USA, Argentina, and Brazil as raw materials, we built the Quantitative Detection for CP4 EPSPS protein of glyphosate-resistant, Genetically Modified Soybean processed by ELISA method. It was detected that genetically modified soybean from USA soybean was 3. 768%, 2. 820% for the Argentina group, and was 1. 920% for the Brail group. CP4 EPSPS protein was not detected in genetically modified soybean power, meal or Chinese soybean. The method was not only simple, rapid, steady but also has good sensitivity level equals to or higher than 0. 0075%. It could serve as an effective method for quantitative detection of the CP4 EPSPS protein of glyphosate-resistant genetically modified soybean.

关 键 词： 酶联免疫吸附法 转基因大豆 定量检测

领　　域： [农业科学]