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菜心耐Cd性的基因型差异及其机制研究
Genotypic variation of Cd-tolerance and corresponded mechanisms in Brassica parachinensis

作  者: ; ; ; ; ;

机构地区: 广西大学农学院

出  处: 《广西农业生物科学》 2007年第4期322-325,330,共5页

摘  要: 采用水培试验方法,研究不同品种菜心(31号、四九、石排)耐镉(Cd)能力的差异及其机制。结果表明,镉(1、3、6μg/mL)抑制菜心生长,使植株生物产量显著降低。地上部是镉对菜心毒害的主要部位,镉处理后菜心的根/冠比增加。镉处理使不同品种菜心叶片中的镉含量和丙二醛(MDA)含量增加,但不同品种菜心生长受镉影响的程度、叶片中的镉含量、MDA含量及过氧化物酶(POD)、过氧化氢酶(CAT)活性存在明显的基因型差异。镉(3、6μg/mL)处理后,四九菜心的相对生物量、POD活性和CAT活性最高而叶片中镉含量、MDA含量最低;相反,31号菜心的相对生物量、POD活性和CAT活性最低,而叶片中镉含量、MDA含量最高;石排菜心则介于二者之间。这些结果表明,四九菜心是耐镉品种,而31号菜心是对镉较敏感的品种,菜心耐镉性的差异与叶片对镉的积累量及抗氧化酶活性不同有关。 The variation of Cd-tolerance in Brassica parachinensis (cv, No, 31, Sijiu, Shipai) and corresponded mechanisms were studied by hydroponic culture. Cadmium (1, 3, 6 μg/mL Cd^2+) induced a decreases in biomass of B. parachinensis significantly, indicating that Cd inhibited plant growth of B. parachinensis. Upper part was the main part of Cd-toxicity in 17. parachinensis since root/shoot ratio of 17. parachinensis increased under Cd stress. Cadmium resulted in the increases of Cd and MDA content in leaves. However, there was significant genotypic variation in growth, Cd and MDA content, and activities of POD and CAT in leaves among cultivars. The highest relative biomass and activities of POD and CAT were found after the treatment with Cd^2+ (3, 6μg/mL) in Siiiu, while Cd and MDA content in their leave were the lowest. By contraries, underCd stress, No. 31 had the lowest activities of POD, CAT and the highest content of Cd and MDA in the leaves, and Shipai was between in Sijiu and No. 31. These results indicated that Sijiu was Cd-tolerant cultivar, while No. 31 was sensitive one and the difference in Cd acumulation and activity of antioxidant enzymes in leaves would involved in the resistance of 17. parachinensis to Cd toxicity.

关 键 词: 菜心 耐性 差异 机制

领  域: [环境科学与工程]

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