作　　者： ; ; ; ; ;

机构地区： 四川农业大学

出　　处： 《畜牧兽医学报》 2007年第12期1300-1305,共6页

摘　　要： 测定了四川7个地方山羊品种（类群）43个个体的mtDNA控制区全序列，结果表明：山羊mtDNA控制区全序列长度为1212bp或1213bp，A＋T含量（59．9％）明显高于G＋c含量（40．1％）。共检测到74个变异位点，序列均为中性突变，核苷酸多样度为1．686％，这些差异共定义了27种单倍型，单倍型多样度为0．966，遗传多样性较为丰富，品种（类群）间存在不同程度的遗传分化。7个地方山羊品种（类群）间的遗传距离变异范围为0．0017～0．0306。用MEGA软件的NJ法构建单倍型序列的系统发育无根树，结果表明四川地方山羊品种（类群）有两个母系来源，但是否就对应于角笋骨羊（Capra aegagrus）和捻角山羊（Capra falconeri）两个野生祖先，还有待于进一步研究。 Complete sequence of mitochondrial DNA（mtDNA） control region was sequenced in 43 individuals from 7 indigenous goat （Capra hircus） breeds （groups） in Sichuan province. The complete sequence of mtDNA control region was 1 212 or 1 213 base pair, and the content of A＋T （59.9 % ）was higher than that of G＋C（40.1% ）significantly. 74 sites were polymorphic and all of these sequences were neutral mutation. All sequence polymorphic sites defined 27 haplotypes. High nucleotide diversity （1. 868 %） and haplotype diversity （0. 966） were manifested. These results suggested richer genetic diversity within Sichuan indigenous goat populations and genetic divergence among them. The Kimura 2-parameter distance among breeds （groups）varied from 0. 001 7 to 0. 030 6. Phylogenetic unrooted trees constructed with neighbour-joining methods showed two maternal origins in Sichuan indigenous goats. More detailed studies should be continued to indicate whether the two maternal origins are Capra aegagrus and Capra falconeri.

关 键 词： 山羊 控制区 序列变异性 种群遗传结构 系统发育

领　　域： [农业科学] [农业科学]