作　　者： ; ;

机构地区： 华中农业大学食品科技学院食品安全与微生物系

出　　处： 《华中农业大学学报》 1997年第5期367-373,共7页

摘　　要： ４种蛋白酶对黑曲霉、米曲霉原生质体稳定性、再生及融合的研究结果表明：蛋白酶用于原生质体灭活前或后保温均显著降低原生质体数量、同时提高原生质体再生率及灭活原生质体融合指数。蛋白酶安全。 The effects of various proteinases on the storage and regeneration of normal protoplast and fusion of inactivated protoplasts between Aspergillus niger V. Tiegh and Aspergillus oryzae Ahlb were studied. The results showed that both proteinase incubation before and after inactivation reduced protolpast number and increased regeneration frequency of normal protoplast and fusion index of inactivated protoplasts. These effects of proteinase varied with treatment patterns such as incubation duration, proteinase concentration and combination of incubation with inactivation. It was observed that proteinase could replace polyethyleneglycol as a good alternative approach of common fusogen with a higher fusion index for inactivated protoplasts.

关 键 词： 曲霉 蛋白酶 原生质体 再生 融合

领　　域： [轻工技术与工程] [轻工技术与工程]