作　　者： ; ; ; ;

机构地区： 同济大学环境科学与工程学院污染控制与资源化研究国家重点实验室

出　　处： 《环境科学研究》 2007年第6期94-100,共7页

摘　　要： 提取降解2-氯酚(2-chlorophenol,2-CP)的厌氧颗粒污泥在Ni2+冲击前后样品的总DNA,用古细菌特异性引物ARC21F/ARC958R进行16S rDNA的聚合酶链式反应(Polymerase Chain Reaction,PCR)、克隆、测序及序列分析等,研究了Ni2+冲击及恢复性驯化对2-CP驯化后厌氧颗粒污泥中古细菌种群多样性的影响.结果表明:Ni2+冲击对古细菌种群结构影响较大,冲击前后厌氧颗粒污泥中存在共有的古细菌菌种(Methanothrix soehngenii,Methanosaeta concilii及Uncultured archaeon等).冲击后厌氧污泥中新出现的古细菌有Toluene-degrading Methanogenic consortium archaeon M1及Candidatus Methanoregula booneistrain6A8等.经过10 d的恢复性驯化后,厌氧污泥中又出现了新的古细菌Methanobacterium subterraneum及Methanobacterium palustre等.2-CP厌氧降解系统经过Ni2+冲击后,古细菌种群多样性显著增加,系统能较快恢复对2-CP的降解能力. The effects of Ni^2＋ on Archaea community structure in anaerobic granular sludge for 2-ehlorophenol degradation were analyzed by 16S rDNA-based approach. Total DNA was extracted directly from the 2-CP-acclimated anaerobic sludge, the sludge being shocked by Ni^2＋ and the sludge being recovered after shock respectively, and then amplified by polymerase chain reaction （PCR） technique with the specific primer pair ARC21F/ARC958R for Archaea. The positive PCR products were cloned and sequenced. The sequences analysis shows that Archaea community structure shifts obviously after being shocked by Ni2. , and there exist some common Archaea among these sludge, including Methanothrix soehngenii, Methanosaeta concilii and Uncultured archaeon, etc. Some special Archaea are found after being shocked by Ni^2＋ , such as Toluene-degrading Methanogenic consortium archaeon M1 and Candidatus Methanoregula boonei strain 6A8, etc. Compared with the sludge being shocked by Ni^2＋ , some special Archaea appear in the sludge being recovered for ten days, including Methanobacterium subterraneum and Methanobacterium palustre, etc. After being shocked by Ni^2＋ , Archaea community diversity increases and the anaerobic system could recover the ability for 2-CP-degradation quickly.

关 键 词： 氯酚 冲击 厌氧颗粒污泥 克隆 古细菌

领　　域： [环境科学与工程]