作　　者： ; ; ; ; ; ; (徐轶鸣）;

机构地区： 华中科技大学同济医学院公共卫生学院环境与健康教育部重点实验室

出　　处： 《卫生研究》 2007年第6期671-673,共3页

摘　　要： 目的确定高效酞酸酯(PAEs)降解菌的驯化与筛选方法,并对PAEs降解性基因功能片段进行初步研究。方法采用活性污泥曝气与密度梯度驯化方法,以邻苯二甲酸二甲酯(DMP)、邻苯二甲酸二乙酯(DEP)和邻苯二甲酸二丁酯(DBP)为碳源,定时定量逐步提高其浓度,连续驯化8周。平板划线分离纯化细菌后,进行降解试验。然后对高效降解菌进行菌种鉴定并提取降解质粒。结果分离出5株PAEs降解细菌,其中2株对DMP、DEP、DBP(底物浓度均为200mg/L)的3天降解率分别达到99.9%、98.6%、99.2%(4号菌)和87.5%、99.1%、98.3%(1号菌),但均未能提取出质粒。经鉴定,4号菌为多杀巴斯德菌。结论曝气池密度梯度驯化法可以驯化出高效降解菌,其降解基因功能性片段不在质粒上而在基因组DNA上。 Objective To define the method of acclimating isolating and screening of dominant bacteria for phthalic acid esters biodegradation and study their plasmids. Methods Active sludge aeration and method of density gradient were used to acclimate the sludge. The concentration of the carbon source including DMP, DEP and DBP was increased step by step in continuous eight weeks. The biodegradation test on PAEs was conducted after bacteria isolation. The dominant bacterium was identified and its degradation plasmid was extracted. Results Five strains of dominant bacteria were screened. Two strains＇ degradation rates to DMP, DEP, DBP in 7 days could reach to 99.9% , 98.6%, 99.2% （No.4 bacterium） and 87,5 %, 99.1%, 98.3 % （ No. 1 bacterium）, respectively. However, their plasmids could not be found. The bacterium which could have. better degradation efficiency was identified as Pasteurella multocida. Conclusion The dominant bacteria could be got through density gradient acclimatization in the active sludge aeration basin. The biodegradation gene fragment of the two strains could exist in its genome but not in plasmid.

关 键 词： 酞酸酯 生物降解 驯化 多杀巴斯德菌

领　　域： [环境科学与工程] [环境科学与工程]