作　　者： ; ;

机构地区： 咸宁学院医学院

出　　处： 《医学新知》 2007年第3期145-146,149,共3页

摘　　要： 目的研究白细胞介素-1β(interleukin-1β,IL-1β)和白细胞介素-6(interleukin-6,IL-6)对体外培养新生大鼠的大脑皮质、海马谷氨酸(glutamate,Glu)和γ-氨基丁酸(γ-aminobutyric,GABA)能神经元细胞免疫反应的影响。方法采用体外细胞培养方法获得新生大鼠大脑皮质和海马的神经细胞,然后在培养液中分别加入IL-1β、IL-6,在作用12h后,通过免疫细胞化学方法,检测大脑皮质、海马谷氨酸能神经元和GABA能神经元的变化。结果IL-1β、IL-6可引起大脑皮质、海马谷氨酸能神经元数目明显增多,GABA能神经元数目明显减少。结论IL-1β、IL-6可能是通过促进谷氨酸能神经元的功能,抑制GABA能神经元的功能,而使神经元兴奋性升高,从而促进癫痫发生。 Objective To study the effects ofinterleukin-1β or interleukin- 6 on culture in vitro of the glutamate and γ-aminobutyric in cortex and hippocampus of neonate rats. Methods The cortex and hippocampus neurocytes of neonatal rats were cultured in vitro, and IL-1β and IL-6 were administrated in the cultured neurocytes. Effects of IL- 1β and/L-6 on the number of neuron of glutamate and γ-aminobutyric in cortex and hippocampus were detected by means of immunocytochemistry method at 12h following drug application. Results The number of neuron of glutamat in the IL-1β or IL-6 groups were significantly higher than those in the model group, while the number of neuron of γ-aminobutyric in the IL-1β or IL-6 groups were significantly lower than those in the model group. Conclusion IL-1β and IL-6 could promote and induce epilepsy through increasing glutamate and decreasing γ-aminobutyric in the cerebral cortex and hippocampus. The nerve excitation was enhanced and then epilepsy occurred.

关 键 词： 白细胞介素 白细胞介素 神经细胞 谷氨酸 氨基丁酸 免疫细胞化学 癫痫

领　　域： [生物学] [生物学]