作　　者： ; ; ; ; ; ; (赖志勇）;

机构地区： 广东省农业科学院

出　　处： 《华中农业大学学报》 2007年第5期722-726,共5页

摘　　要： 利用高效液相色谱（HPLC）法同时分离测定了龙眼肉中的9种核苷类成分（尿嘧啶、胞苷、尿苷、胸腺嘧啶、次黄嘌呤核苷、鸟苷、胸苷、腺嘌呤相对标准偏差和腺苷）。分离柱为Nucleosil C18反相柱（250 mm×4 mm）,流动相为0.01 mol/L的KH2PO4和甲醇,梯度洗脱,流速1 mL/min,检测波长260 nm,柱温40℃。用外标法测定龙眼肉中9种核苷成分的含量。在供试条件下,选取9种核苷标准品的线性范围为0.05-3.20μg/mL,相关系数r=0.9999-1.0000;标准品在0-48 h内经多次测定,保留时间和峰面积的相对标准偏差分别为0.133%-1.156%和1.153%-3.023%;回收率和回收率相对标准偏差分别为98.79%-107.34%和1.411%-4.544%;仪器最低检出限分别为0.0035-0.0136μg/mL。 Nine nueleosides （uracil, cytidine, uridine, thymine, inosine, guanosinc, thymidine, adenine and adenosine） in flesh of Dimocarpus longan Lout. were well ,separated and determined by HPLC. Separations were carried out in reversed-phase Nucleosil Cls column（250 min×4 mm） by using mobile phase 0.01 mol/L KH2PO4 CH3OH at detection wavelength 260 nm and column temperature 40℃. Gradient elution proceeded with the flow rate of 1 mL/min. External calibration was used for nucleosides determinations. Results showed that linear calibrations of nine standard nucleosides were within 0.05-3.20 μg/mL with correlation 0. 999 9- 1. 000 0. RSD of retained time and peak area were respectively within 0. 133%-1. 156% and 1. 153%-3. 023% after standard samples were repeated analyzed in 48hrs. Recovery was within 98. 79%-107.34% with RSD of 1. 411%-4. 544%. Detection limits were within 0. 003 5-0. 013 6 μg/mL.

关 键 词： 高效液相色谱 龙眼 核苷

领　　域： [农业科学] [农业科学]