作　　者： ; ; ; (姚程炜）;

机构地区： 绵阳师范学院化学与化学工程学院

出　　处： 《应用化学》 2007年第11期1343-1345,共3页

摘　　要： 使用自行设计的毛细管电泳柱端电导检测系统，对甲霜灵对映体进行了拆分。着重研究了缓冲溶液浓度和酸碱度、手性拆分剂（β-CD）分离高压、进样时间等因素对分离的影响。以无涂层融硅石英毛细管（75μm×50cm）为分离柱，以5．0mmol／L Na2B4O7＋2．0mmol／L H3BO3为缓冲溶液（pH=9．0），分离电压为15 kV，R体在5．0—120．0mg／L范围内峰高（y）与质量浓度（x）间呈良好的线性关系，线性系数R=0．9982，线性回归方程为：Y=6．1＋24．3x，检出限为1．5mg／L；S体在10．0．150．0mg／L范围内峰高（y）与质量浓度（x）间呈良好的线性关系，R=0．9986，其线性回归方程为：y=4．1＋18．9x，检出限为5．0mg／L。 A separation method for Metalaxyl enantiomers by capillary electrophoresis with conductive detection was developed. The effect of running buffer, pH, the concentration of the chiral selector β-CD, separation voltage and injection time were discussed in detail. Under the following separation conditions： uncoated fused silica capillary （45 cm × 75 μm ） as separation channel, 5 mmol/L Na2 B4O7. H2O-2 mmol/L H3 BO3- 4 mmol/L β-CD（ pH = 9.0 ） as running buffer, and 15 kV separation voltage, linear correlation was found between the peak height （y） and the mass concentration of R-Metalaxyl （x） in the range of 5.0 120.0 mg/L, with the regression equation y = 6. 1 ＋ 24.3x, and the correlation coefficient was 0. 998 2, and the determination limit was 1.5 mg/L; linear correlation was found between the peak height and the mass concentration of S-Metalaxyl（x） in the range of 10. 0 - 150. 0 rag/L, with the regression equation y =4. 1 ＋ 18.9x, and the correlation coefficient was 0. 998 6, and the determination limit was 5.0 mg/L.

关 键 词： 毛细管电泳 甲霜灵 对映体拆分 电导检测

领　　域： [理学] [理学]