作　　者： ; ; ; ;

机构地区： 华南师范大学体育科学学院

出　　处： 《体育学刊》 2007年第7期57-60,共4页

摘　　要： 为探讨低氧以及运动对肥胖和正常大鼠骨骼肌线粒体脂质过氧化、抗氧化能力的影响。将100只健康雄性SD大鼠随机分为正常对照组(40只)和肥胖造模组(60只),并从造模成功的大鼠中挑选40只,随机分为肥胖常氧安静组、肥胖常氧运动组、肥胖低氧安静组和肥胖低氧运动组(每组10只)。正常对照组随机分为正常常氧安静组、正常常氧运动组、正常低氧安静组、正常低氧运动组,每组10只。第4周末次低氧运动后24 h左右进行采样,采样前所有大鼠禁食过夜,取后肢骨骼肌(腓肠肌、股四头肌)匀浆提取线粒体。测定肥胖组大鼠以及正常组大鼠骨骼肌线粒体MDA含量及SOD活性。结果:(1)正常组大鼠骨骼肌线粒体SOD活力明显高于造模组大鼠(P<0.01);正常组大鼠骨骼肌线粒体MDA含量低于造模组大鼠,但没有统计学意义。有氧运动组大鼠骨骼肌线粒体SOD活力高于安静组,而MDA含量则低于安静组,但无统计学意义。(2)低氧安静组大鼠骨骼肌线粒体SOD活力明显高于常氧安静组(P<0.01);MDA含量低于常氧安静组,但无统计学意义。(3)低氧运动组大鼠骨骼肌线粒体SOD活力明显高于常氧运动组(P<0.05),MDA含量则低于常氧运动组,但无统计学意义。结果表明,肥胖鼠机体的抗自由基能力比正常鼠差,而运动和低氧刺激能改善这种状况;4周的有氧运动以及低氧刺激使机体的抗氧化能力增强,自由基清除能力提高;运动和低氧刺激相结合能使机体的抗氧化能力和自由基清除能力更强。 In order to probe into the effects of hypoxia and exercising on the super-oxidizing and oxidation resisting capability of the lipid of mitochondria of skeletal muscles （MSM） of fat and normal rats, the authors randomly divided 100 healthy male rats into a normal control group of 40 rats and a group of 60 rats for fat model establishment, selected 40 rats from the fat model successfully established Sprague Danley rats and randomly divided them into a normoxic resting group, a normoxic exercising group, a hypoxic resting group and a hypoxic exercising group （each group contains 10 rats）, divided the control group into a normal normoxic resting group, a normal normoxic exercising group, a normal hypoxic resting group and a normal hypoxic exercising group （each group contains 10 rats）, sampled the homogenate of skeletal muscles （gastrocnemius and quadriceps femoris） of the posterior limbs of the rats to extract mitochondria in about 24 h after the last hypoxic exercising was performed in week 4 （all the rats were starved at the night before sampling）, and tested the MDA content and SOD activity of MSM of rats in the fat model established groups and normal groups. The authors revealed the following findings：（l） as for MSM of rats in the normal groups, their SOD activity is significantly higher than that of rats in the fat model established groups （P〈0.01）, while their MDA content is lower than that of rats in the fat model established groups, but which is not significant statistically; as for MSM of rats in the aerobic exercising groups, their SOD activity is higher than that of rats in the resting groups, while their MDA content is lower than that of rats in the resting groups, but those are not significant statistically; （2） as for MSM of rats in the hypoxic resting groups, their SOD activity is significantly higher than that of rats in the normoxic resting groups （P〈0.01）, while their MDA content is lower than that of rats in the normoxic resting groups, but which is not si

关 键 词： 运动生物化学 肥胖大鼠 间歇性低氧 有氧运动 自由基 线粒体

领　　域： [文化科学] [文化科学]