作　　者： ; ; ; ; ;

机构地区： 暨南大学生命科学技术学院生物医学工程系

出　　处： 《第四军医大学学报》 2007年第20期1862-1864,共3页

摘　　要： 目的:探讨900MHz电磁波对淋巴细胞的影响.方法:利用显微激光共焦拉曼光谱技术获得功率密度为5mW/cm2的900MHz电磁波照射不同时间的淋巴细胞的拉曼光谱.结果:照射20min后,部分淋巴细胞拉曼光谱出现了微小变化;照射40min后,包含类胡萝卜素信息的细胞核拉曼光谱出现明显变化,细胞核的拉曼光谱的一些谱带也发生了变化;照射60min后,淋巴细胞拉曼光谱位于787cm-1的谱线移动了8cm-1,显示细胞核受到电磁辐射后产生明显的损伤.结论:长时间处于5mW/cm2电磁波作用下(≥40min),淋巴细胞出现损伤,损伤主要表现在蛋白构象变化和DNA的碱基受到损伤,从而影响其功能. AIM： To study the effect of 900 MHz electromagnetic fields on the lymphocytes at molecular level. METHODS： The lymphocytes exposed to 900 MHz electromagnetic fields with power density of 5 mW/cm^2 were measured by confocal Raman microspectroscopy technique. RESULTS ： After 20 min exposure, Raman spectra of some lymphocytes changed a little. After 40 min, obvious difference appeared in the Raman spectra of the nucleolus either with the information of carotenoids or without. After 60 min exposure, the Raman band at 787 cm^-1 shifted 8 cm^-1, indicating that there was obvious damage in the lymphocytes. CONCLUSION： The exposure of lymphocytes to 900 MHz electromagnetic fields of 5 mW/cm^2 for a period of time （ ≥40 min） would induce damage in lymphocytes, including the change of protein structure and the damage of DNA base conformation, which suggests that the function of lymphocytes would be affected by the electromagnetic fields.

关 键 词： 电磁波辐射 淋巴细胞 光谱分析 拉曼

领　　域： [理学] [理学]