作　　者： ; ; ;

机构地区： 第四军医大学口腔医学系

出　　处： 《临床口腔医学杂志》 2007年第10期585-587,共3页

摘　　要： 目的:通过对大鼠骨髓基质干细胞成脂肪分化过程中雌激素受体β表达规律的研究,进一步明确雌激素受体β与骨代谢的相关性。方法:大鼠骨髓基质干细胞培养,成骨诱导分化,钙染色。成脂肪诱导分化,油红O染色。Western blot方法测定大鼠骨髓基质干细胞成脂肪分化过程中雌激素受体β的表达。结果:全骨髓贴壁方法培养获得大鼠骨髓基质干细胞。成脂诱导分化显微镜下脂肪滴、油红O染色阳性细胞随成脂肪时间增加而增多。雌激素受体β的表达随成脂时间的增加而递增。结论:大鼠骨髓基质干细胞成脂肪分化过程中ERβ的表达随时间逐渐增加。 Objective：To study the regulation of estrogen receptor expression β Sprague Dawleg rats marrow mes- enchymal stem cells during differentiating into adipogenic in vitro.To elucidate the mechanism of the role of estrogen recep- tor β in bone metabolism. Method： Sprague Dawleg rats marrow mesenchymal stem cells were separated and cultivated. To induce the rBMSCs to adipogenic in vitro. ERβ protein was examined by Western blot in different stage. Result： The cells cultured were identified as marrow mesenchymal stem cells.The＇protein stripes in Western blot of ERβ were clearer with time adding during differentiating adipogenic. Conelution： ERβ protein in Sprague Dawleg rats marrow mesenchymal stem cells were high with the time adding during differentiating adipogenic.

关 键 词： 雌激素受体 骨髓基质干细胞 成脂肪分化

领　　域： [生物学]