作　　者： ; ; ; ; ; ;

机构地区： 广东省农业科学院

出　　处： 《广东农业科学》 2007年第10期27-29,共3页

摘　　要： 采用高效液相色谱法(分离柱为C18反相柱、柱温40℃,流动相为甲醇∶水=36∶64、流速为0.8 mL/min,紫外检测器的检测波长为233 nm,外标法定量)测定了开花坐果期荔枝叶片中内源多胺Put、Spd、Spm的含量,结果表明:Put、Spd和Spm标准品保留时间分别为6.648、8.892、12.246 min,一次样品的测定可在15 min内完成;Put、Spd和Spm在0.03125～2 nmol线性范围内,相关系数分别为0.9996、0.9996、0.9995;精密度和稳定性试验的相对标准偏差均小于5%,说明该测定方法稳定、分析精密度高。 Putrescine （Put）, spermidine （Spd） and spermine （Spm） contents in litchi （Litchi chinensis） were analysed during blooming and fruiting by HPLC. By using the C18 reverse phase column at 40℃,the flow phase rate of Methanol：Water was 36： 64, at a flow rate of 0.8 mL/min , and with a UV detector at 233 nm. Quantities were calculated with external standards.The results showed that the retain time of Put, Spd and Spm was at about 6.648, 8.892 and 12.246 minutes, respectively. The analysis could be finished in 15 minutes. All the relative coefficients were above 0.999 when the linear arrange of the three polyamines was from 0.03125 to 2 nmol. The relative standard deviation of precision and stability experiments was lower than 5%, indicating that the method was a simple and rapid method with more accuracy and stability.

关 键 词： 荔枝 内源多胺 高效液相色谱分析

领　　域： [农业科学] [农业科学]