作　　者： ; ; ; ; ; ;

机构地区： 河北农业大学植物保护学院河北省农作物病虫害生物防治工程技术研究中心

出　　处： 《农药学学报》 2007年第3期269-274,共6页

摘　　要： 嗜线虫致病杆菌HB310(Xenorhabdus nematophila HB310)菌液中主要的杀虫活性物质是一种高分子量的复合蛋白-毒素Ⅱ。以该菌液和毒素Ⅱ分别饲喂棉铃虫Helicoverpa armigera幼虫,检测其对棉铃虫生长发育的影响,同时通过生化分析研究了该毒素对幼虫中肠内几种蛋白酶活力的影响。结果表明:菌液和毒素Ⅱ对棉铃虫幼虫的取食量和生长发育均有显著的影响,取食拌有菌液和毒素Ⅱ的人工饲料的棉铃虫食量明显减少,发育速度延缓,发育历期比对照明显推迟;尽管一直取食混合原菌液(6.5×108cells/mL)人工饲料的棉铃虫2龄幼虫前期死亡率很低,但是其生长发育几乎完全被抑制,该处理组所有幼虫均不能化蛹;原菌液对4龄幼虫的食量、发育历期、蛹重及化蛹率均有显著影响。菌液对棉铃虫幼虫的影响与菌液的浓度和幼虫的龄期成反比,稀释50倍的菌液对2龄和4龄幼虫的生长发育仍有一定影响;毒素Ⅱ(51.9μg/mL)对4龄棉铃虫的生长发育也有明显的抑制作用。短时间(2d)饲喂原菌液后更换正常饲料,仅延缓了棉铃虫幼虫的发育历期,而对其化蛹率、蛹重及羽化率均无明显影响。饲喂毒素Ⅱ的棉铃虫幼虫中肠主要蛋白酶的活性受到明显的抑制。 The main insecticidal toxin from Xenorhabdus nematophila HB310 was a kind of high molecular weight protein complex--toxin Ⅱ. The effects of the culture broth and the toxin Ⅱ on the growth and several midgut enzymatic activities of the H. armigera larvae were investigated. The results indicated that the culture broth and the toxin Ⅱ not only affected the feeding food quantity but also disrupted the development of the H. armigera larvae （ including larvae period, pupation percentage, pupa weight and emergence percentage ）. The growth of the 2nd instar larvae exposed to 6. 5 × 108 cells/mL culture broth was almost entirely inhibited, and all the larvae did not pupated. The growth of the 2nd instar larvae exposed to 1.3× 107 cells/mL culture broth was still markedly inhibited. The growth of the 4 th instar larvae exposed to 51.9 μg/mL toxin Ⅱ was also inhibited remarkably. Short time exposure to the culture broth prolonged the development period of the 2rid and 4th instar larvae, but did not impact on the pupation percentage, pupa weight and emergence percentage of H. armigera. Toxin Ⅱ also significantly inhibited the primary enzymatic activities of the midgut of H. armigera larvae.

关 键 词： 嗜线虫致病杆菌 棉铃虫 蛋白酶 中肠 生物活性

领　　域： [农业科学] [农业科学] [农业科学] [农业科学]