作　　者： ; ; ;

机构地区： 仲恺农业技术学院

出　　处： 《湖北农业科学》 2007年第5期674-676,共3页

摘　　要： 对转基因植株T0代种子进行发芽试验以及对T1代植株叶片进行浸泡试验,以研究转基因植株后代的遗传及表达情况。结果表明,转基因植株T0代种子在含50 mg·L-1潮霉素的培养基上正常发芽生长,而对照虽能发芽,但最终枯黄死亡;T1代植株叶片在100 mg·L-1潮霉素溶液中浸泡3 d后,部分叶片变黄,部分叶片仍为绿色,统计分析表明,转基因植株外源片段为单拷贝整合;对潮霉素浸泡后的植株的PCR分析表明,潮霉素浸泡对后代的遗传分析是行之有效的。 For genetic analysis of transformants, the To generation seeds of the transgenic plants were germinated and the leaves of T1 generation plants were soaked. The result indicated： the seeds of the transgenic plants could germinate and grew very well on the MS medium with 50mg·L^-1 hygromycinB. The seeds of the non-transgenic plants also could germinate, but its seedlings wilted and died finally; the segregation analysis of Hyg-resistant in T1 progeny showed the foreign fragments was one locus; soaking the leaves with hygromycin B was proved to be an useful method for genetic analysis of progeny by the correspondent PCR analysis.

关 键 词： 转基因 水稻 分子检测 后代分析

领　　域： [农业科学] [生物学]