作　　者： ; ; ; ; ; ; ;

机构地区： 华南理工大学化学与化工学院化学系

出　　处： 《高等学校化学学报》 2007年第9期1628-1630,共3页

摘　　要： Several Mn(Ⅲ)10-(hydroxylphenyl)-5,15-bis(pentafluoro-phenyl)corroles with the hydroxyl at ortho-,meta-and para-position of 10-phenyl group were used as the catalysts in the oxidative cleavage of plasmid DNA in the presence of H2O2.The catalytic system was composed of 10 μL 0.5 mg/mL pC DNA 6 in TE buffer solution(pH=8.0,4.0×10-2 mol/L Tris,1.0×10-3 mol/L EDTA),10 μL 1.0×10-5 mol/L Mn(Ⅲ)corrole in DMSO and 10 μL 0.3% H2O2.After 8 h incubation at room temperature,significant oxidative DNA damage could be observed with the ortho-hydroxyl Mn(Ⅲ)corrole showing a better activity as detected by agarose-gel electrophoresis.Plasmid DNA was completely damaged after 12 h incubation.These observations show Mn(Ⅲ)corrole can be used as an artificial model of nuclease. Several Mn（ Ⅲ） 10-（hydroxylphenyl）-5,15-bis（pentafluoro-phenyl） corroles with the hydroxyl at ortho-, meta- and para-position of 10-phenyl group were used as the catalysts in the oxidative cleavage of plasmid DNA in the presence of H202. The catalytic system was composed of 10 μL 0. 5 mg/mL pC DNA 6 in TE buffer solution（pH =8.0, 4.0 ×10^-2 mol/L Tris, 1.0 ×10^-3 mol/L EDTA）, 10 μL 1.0 ×10^-5 mol/L Mn（ Ⅲ） corrole in DMSO and 10 μL 0.3% H2O2. After 8 h incubation at room temperature, significant oxi- dative DNA damage could be observed with the ortho-hydroxyl Mn （Ⅲ ） corrole showing a better activity as detected by agarose-gel electrophoresis. Plasmid DNA was completely damaged after 12 h incubation. These observations show Mn（Ⅲ） corrole can be used as an artificial model of nuclease.

关 键 词： 锰 氧化断裂

领　　域： [理学] [理学]