作　　者： ; ; ; ; ;

机构地区： 华南农业大学资源环境学院农药与化学生物学教育部重点实验室

出　　处： 《南京农业大学学报》 2007年第3期 135-138,共4页

摘　　要： 从7．920％印楝素A干粉中分离出印楝素B，并对其结构进行了鉴定。生物活性测定结果表明，印楝素B对斜纹夜蛾幼虫具有较好的拒食活性，处理后24h，印楝素B对斜纹夜蛾2龄幼虫的非选择性拒食中浓度（AFC50）为2．42μg·mL^-1；处理后24h和48h，对斜纹夜蛾3龄幼虫的选择性AFC50分别为0．18和0．22μg·mL^-1。印楝素B对大菜粉蝶幼虫具有一定的拒食活性，处理后24h，对大菜粉蝶幼虫非选择性AFC50为224．48μg·mL^-1。印楝素B对赤拟谷盗具有较好的忌避活性，处理后24h，50、30、10μg·mL^-1的印楝素B对赤拟谷盗的平均忌避率分别为86．24％、85．06％和70．83％；处理后48h，其平均忌避率分别为67．70％、59．49％和59．74％。 Azadirachtin B was isolated from azadirachtin A-rich dried powder that contained 7.920% azadirachtin A. The structure of azadirachtin B was identified. AFC5o （media antifeedant concentration） in non-choice condition of azadirachtin B against 2nd instar larvae of Spodoptera litura was 2.42 μg·mL^-1 treatment after 24 h. AFC50 in choice condition of azadirachtin B against 3rd instar larvae of S. litura were 0. 18 μg·mL^-1 and 0.22 μg·mL^-1 treatment after 24 h and 48 h. AFC50 in non-choice condition of azadirachtin B against 3rd instar larvae of Pieris brassicae was 224. 48 μg·mL^-1 treatment after 24 h. Average deterrent rates of 50 μg·mL^-1, 30 μg·mL^-1 and 10 μg·mL^-1 azadirachtin B against Tribolium castaneum were 86.24%, 85.06% and 70. 83% treatment after 24 h, and were 67.70%, 59.49% and 59.74% treatment after 48 h.

关 键 词： 印楝素 分离 结构鉴定 生物活性

分 类 号： [S482.39]

领　　域： [农业科学] [农业科学]