机构地区: 南方医科大学公共卫生与热带医学学院环境卫生学系
出 处: 《现代生物医学进展》 2007年第9期1308-1309,1312,共3页
摘 要: 多环芳烃是一类普遍存在的环境污染物。本研究探讨了普通富集法,固定化富集法以及巴斯德消毒后富集法三种途径从相同红树林土壤中分离菲降解茵的差异。通过平板培养和变性梯度凝胶电泳两种方法分析分离结果。上述方法分别获得以鞘氨醇单胞茵、分枝杆菌以及红球茵为优势菌群的群落,表明分离方法对多环芳烃降解菌多样性的研究是一种重要的影响因素。 Polycyclic aromatic hydrocarbons are a group of ubiquitous pollutants. The present study compared the isolation of phenanthrene-degrading bacteria from mangrove sediment by traditional, immobilized and pasteurized enrichment methods. Cultivation on agar plates and denaturant gradient gel electrophoresis data showed that the three methods resulted in the isolation of Sphingomonas, Mycobacterium and Rhodococcus strains, respectively, indicating that the enrichment method was critical for the study of diversity of polycyclic aromatic hydrocarbon-degrading bacteria.
关 键 词: 多环芳烃 富集法 鞘氨醇单胞菌 分枝杆菌 降解
领 域: [生物学]