作　　者： ; ; ; ; ; ; ; ; (李邦佑）; (王定发）;

机构地区： 广东省农业科学院

出　　处： 《华中农业大学学报》 2007年第4期528-532,共5页

摘　　要： 在纯化日粮中分别添加0、60、120、180、240和300 mg/kg还原型谷胱甘肽(GSH),饲喂平均体重为(1.12±0.01)g的凡纳滨对虾(Litopenaeus vannamei)8周,观察饲料中不同浓度谷胱甘肽对凡纳滨对虾非特异性免疫因子和部分相关酶活性的影响。结果表明:随着饲料中GSH添加量的增加,凡纳滨对虾的髓过氧化物酶(MPO)、溶菌酶(LSZ)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)活性值均呈先升后降的趋势,在120～240 mg/kg时达到最高值,且显著高于对照组;随着饲料中GSH添加量的增加,血清和肝胰腺中的谷草转氨酶(AST)和谷丙转氨酶(ALT)呈下降趋势,在240 mg/kg组达到最低,且显著低于对照组,但在300 mg/kg组又有所回升。以上研究表明,饲料中添加一定浓度的GSH能有效提高凡纳滨对虾血清和肝胰腺中髓过氧化物酶、溶菌酶和磷酸酶活性,并影响转氨酶的活性,从而激发了凡纳滨对虾的非特异免疫功能。 The study was aimed at determining if the dietary supplementation of GSH in Litopenaeus vannamei could affect its nonspecific immune factors and the activities of AKP, ACP, AST and ALT. The shrimp Litopenaeus vannamei at the initial body weight of （1.12 q-0. 01） g were fed diets respectively supplemented with 0,60,120,180,240 and 300 mg/kg diet GSH for 8 weeks. 15 of each repetition were sampled to analyze the immune factors＇ activity of MPO, LSZ, AKP, ACP, AST and ALT in serum and hepatopancrea. The results showed that, in hepatopancrea, with the increasing of dietary GSH level, activities of MPO,LSZ,AKP and ACP of the shrimp increased, reaching the highest level at G120 ,G240, G240 ,G120 respectively, then significantly decreased at a higher level. While in serum, activities of LSZ, AKP and ACP increased, reaching the highest level at G180, G240 and G240 respectively, then significantly decreased. As for AST and ALT （both in serum and hepatopancrea） of shrimp fed dietary GSH, the activities decreased significantly then increased at G240 and G300. The above results would illuminate that the dietary supplementation of GSH at a suitable level could improve nonspecific immunity of the shrimps and affect the activities of AKP, ACP, AST and ALT.

关 键 词： 凡纳滨对虾 谷胱甘肽 非特异免疫因子 转氨酶

领　　域： [农业科学] [农业科学] [农业科学]