作　　者： ; ; ; ; ; ; ;

机构地区： 上海师范大学生命与环境科学学院

出　　处： 《光谱学与光谱分析》 2007年第8期1519-1522,共4页

摘　　要： 傅里叶变换红外光谱结合多元统计方法应用于微生物的快速鉴定及分类是近几年发展起来的一门新型技术。文章用傅里叶变换红外光谱研究了镰刀菌属五种6株镰刀菌。在获得分辨率高、重现性好的镰刀菌全细胞组分的红外谱图基础上,分析了主要吸收峰的归属,确定了镰刀菌红外谱图3000-2800cm^-1,1800-1600cm^-1与指纹区3个分析灵敏区。根据分析灵敏区的差异性区分了不同种的镰刀菌,并在此基础上进行了系统聚类分析并对镰刀菌种间进化亲缘关系进行了比较。结果表明,可根据光谱峰位、峰值及吸收强度比鉴别镰刀菌,而系统聚类分析方法能更科学合理地鉴别镰刀菌及呈现种间相似性程度,但不适合反映镰刀菌遗传亲缘关系。傅里叶变换红外光谱技术所具有快速、精确,易操作等优点是必将成为微生物研究领域的一个重要工具。 Fourier transform infrared （FTIR） spectroscopy, in combination with multi-variate statistics, has been a novel tool to identify and classify microorganisms in recent years. In the present study, Fourier transform infrared spectroscopy was used to identify Fusarium belonging to F. arysporum , F. monil i f orme , F. graminearum , F. semitectum , and F. nivalee . Distinct spectral differences among these strains were observed from their high-resolution and well-reproducibility infrared spectra in the frequency regions of 3 000-2 800 cm^-1 , 1 800-1 600 cm^-1 , and fingerprint region. According to the characteristic bands in these regions, different Fusarium species could be identified. And identification by hierarchical cluster analysis was more scientific and reasonable and it also presented a degree of comparability among these strains. But the result was not becoming on reflecting the characteristic of cladistics. FTIR shows high potential as a rapid, accurate and easy-to-use method for the identification of microorganisms. It will be an important tool in microbiological studies.

关 键 词： 镰刀菌 傅里叶变换红外光谱 鉴别

领　　域： [理学] [理学]