作　　者： ; ; ; ; ;

机构地区： 广东海洋大学水产学院

出　　处： 《水生生物学报》 2007年第4期479-484,共6页

摘　　要： 饵料是鱼类早期发育最重要的条件之一,并且随着个体发育,饵料类别不断变化。为了评定各种饵料的效果并筛选得到军曹鱼仔鱼早期发育阶段适宜的饵料组合,进行了本实验。军曹鱼仔鱼在750L桶中孵出并暂养2d,然后在水泥池中培育4d(3—6日龄),并投喂轮虫;在第7日龄,将240尾仔鱼随机平均分配至12个70L桶中,共分4组,每组3桶;7—15日龄,4组仔鱼分别投喂轮虫、卤虫无节幼体、桡足类和桡足类投;16—42日龄,分别投喂轮虫、卤虫无节幼体、桡足类和鱼糜。第42日龄,将全部仔鱼取样、称重并于-80℃保存备用。鱼体匀浆、离心后的上清液作为粗酶液用以测定蛋白酶、淀粉酶和脂肪酶等消化酶活力,还测定了粗蛋白、粗脂肪等主要体成分,并统计了生长指标。结果表明:(1)组IV的生长表现最佳;(2)组III的成活率最高;(3)饵料对三种酶的活力和体成分都有极显著的影响;(4)组III的蛋白酶活力、组I的淀粉酶活力和脂肪酶活力最高,尤其是组I的淀粉酶活力比其他各组高出6.8—11.9倍;(5)各组鱼体的粗蛋白含量均较相应饵料的低,而粗脂肪含量则比饵料的高。因此,可以认为:仔鱼长期饥饿时,消化酶可能存在补偿性分泌,并优先利用糖类和脂肪作为能源;军曹鱼仔鱼对脂肪的沉积效率较蛋白高。 Diet is one of the most important conditions during fish early stage especially for marine fishes, and it changes continuously as the fish develops. The experiment was conducted to evaluate the effect of different diets on cobia larvae and acquire the optimal diet combination. The eggs were hatched in a 750L tank in which larvae were stocked for 2 days, then larvae were cultured in a concrete pool on for g days （3-6DAH）, feeding on rotifers. On 7DAH, 240 larvae were transferred randomly to twelve 70L tanks with 20 larvae per tank labeled group Ⅰ, group Ⅱ, group Ⅲand group Ⅳ（3 tanks per group）. During 7-15DAH, the larvae were fed with rotifers, artemia nauplii, copepods and copepods respectively; during 16-42DAH, with rotifers, artemia nauplii, copepods and fish meal respectively. On 42DAH, all larvae were sampled, weighed and store in - 80℃ . Crude digestive enzymes were produced from the supernatant by centrifuging homogenized solution of the fish bodies, and the activities of protease, amylase and lipase were analyzed. At the same time, body composition, as well as growth indices of larval cobia were determined. The results showed that： （1） the growth performance of group Ⅳwas the best; （2） the survival of group Ⅲwas the highest; （3） the activities of digestive enzymes and body composition were extremely affected by diet; （4） the protease activity of group Ⅲ, amylase and lipase activity of group Ⅰwas the highest respectively among the 4 groups, and group I was highlighted for its 6.8-11.9 times higher amylase activity than other groups; （5） the crude protein contents of fish bodies were lower than them of corresponding diets, on the contrary, crud fat contents of fish bodies were higher than them of diets. So, there might be compensatory secretion of digestive enzymes, and glucide ＆ fat were utilized preferentially for chronically hungered larvae with fat being more effectively deposited than protein for larval cobia.

关 键 词： 饵料 军曹鱼仔鱼 生长 消化酶 体成分

领　　域： [农业科学] [农业科学]