作　　者： ; ; ; ;

机构地区： 南方医科大学

出　　处： 《中国实验诊断学》 2007年第7期908-911,共4页

摘　　要： 目的运用实时荧光定量RT-PCR的方法,检测小RNA分子(small interfering RNAs,siRNA)对乙型肝炎病毒(hepatitis B virus,HBV)复制的抑制作用。方法在HepG2.2.15细胞内导入筛选的三条特异抗HBV的siRNA分子,用实时荧光定量RT-PCR的方法,检测干扰后HBV的mRNA表达水平。结果导入特异siRNA分子的细胞中HBV的mRNA表达量明显降低。结论实时荧光定量RT-PCR技术能准确可靠的检测mRNA的表达水平,对siRNA分子干扰效果的评价有很好的应用价值。 Objective To use real-time fluorescence quantitative RT-PCR technology, then detecting the interfering effect of siR- NA in HBV replication.Methods Three siRNA sequences were transfected into HepG2.2.15 cells. The amount of HBV mRNA in the HepG2.2.15 cells after transfection was quantitated by a real-time fluorescence quantitative RT-PCR assay. Results There were a decrease in the levels of HBV mRNA from the the transfected cells. Conclusion Real-time fluorescence quantitative RT-PCR technology could detect the mRNA level accurately,it is of good practical value in the detecting interfering effect of siRNA.

关 键 词： 小干扰 分子 乙型肝炎病毒 细胞 实时荧光定量 技术

领　　域： [生物学] [生物学]