作　　者： ; ; (姚程炜）;

机构地区： 绵阳师范学院化学与化学工程学院

出　　处： 《应用化学》 2007年第3期327-330,共4页

摘　　要： 建立了鱼类中孔雀石绿残留量的高效毛细管电泳-电导法。探讨了缓冲溶液浓度、pH值及其分离电压,进样时间,有机添加剂对检测的影响。以无涂层融硅石英毛细管（75μm×50cm）为分离柱,乙酸-乙酸铵（乙酸铵浓度为12mmol/L）为电泳介质,异丙醇为有机添加剂,分离高压为20kV,pH=4.0条件下,在7.2-180mg/L范围内峰高（y）与质量浓度（c）之间呈良好的线性关系,线性系数R=0.9962,线性回归方程为：y=2.02＋102c,检出限为1.8mg/L,样品加标加收率在90%-102%之间。结果表明,该法简便、快速、准确,回收率较高。 A method for determination of Malachite green in fishes was established by high performance capillary electrophoresis using self-constructed conductivity detection system. Several parameters affecting the determination were studied, including concentration of buffer, value of pH, organic concentration, separation voltage, and sampling time. Under the following condions：uncoated fused silica capillary（45 cm× 75 μm） as separation channel, HAC-NH4AC（concentration of NH4AC 12 mmol/L, pH =4.0） as running buffer, 20 kV as separation voltage, a linearity exists between peak height（y） and mass concentration of malachite green（c） in the range of 7.2 - 180 mg/L with regression equation y = 2.02 ＋ 102c, linearity coefficient is 0. 996 2, determination limit is 1.8 mg/L and average recovery rate is from 90. 0% - 102.0%. The results show that the method is simple, quick, accurate and has high recovery.

关 键 词： 毛细管电泳 孔雀石绿 电导检测

领　　域： [理学] [理学]