作　　者： ; ; ; ;

机构地区： 汕头大学理学院生物系

出　　处： 《细胞生物学杂志》 2007年第3期425-428,共4页

摘　　要： 用不同浓度的地鳖虫蛋白粗提物（0．2～0.8g／ml）作用于S180肉瘤荷瘤小鼠，观察各组的抑瘤效果及重要生理指标的差异；同时分别用地鳖虫蛋白粗提物以及经过盐析、离子交换层析、分子筛由地鳖虫蛋白粗提物分离纯化得到的活性蛋白，作用于鸡胚尿囊膜，观察它们对新生血管生成的抑制作用。结果显示，地鳖虫蛋白粗提物对S180肉瘤荷瘤小鼠有显著的抑瘤作用；蛋白质粗提物以及纯化得到的活性蛋白对鸡胚尿囊膜新生血管的生成有明显的抑制作用，纯化品的抑制活性高于粗品，且二者对鸡胚生长发育的影响较阳性对照（地塞米松组）小，差异显著。因此，地鳖虫蛋白提取物有良好的体内抑瘤作用及血管生成抑制活性。 This study was designed to evaluate the activities of anti-angiogenesis and anti-tumor with the protein extractions from Eupolyphaga sinensis, a kind of Chinese traditional medicine. The mice with S 180 sarcoma inoculated were treated （i.g.） with the crude protein extractions at three different doses （0.2 g/ml, 0.4 g/ml, 0.8 g/ ml, as crude materials）, then the inhibitory rate of tumor growth and some physical indexes of antioxidation of the mice were taken. The tumors peeled off the mice which were treated with the crude protein extractions were suppressed remarkably. Chick embryo chorioallantoic membrane （CAM） assay was used to determine the neovascularizative effect in vivo with the crude proteins extractions and the groups purified by ammonium sulphate precipitation, DEAE-cellulose and Sephadex G-75 Column chromatography, and the potent activities of anti-angio- genesis were exhibited respectively. All of our data suggest that the proteins extracted from Eupolyphaga sinensis with fibrinolytic activity must contain the potency to inhibit tumor growth and angiogenesis in vivo.

关 键 词： 地鳖虫 血管生成 抗肿瘤

领　　域： [农业科学] [农业科学]