作　　者： ; ; ; ; (刘华）;

机构地区： 同济大学环境科学与工程学院污染控制与资源化研究国家重点实验室

出　　处： 《环境污染与防治》 2007年第6期406-410,414,共6页

摘　　要： 从多处受石油污染的土壤中经过初步筛选、混合驯化得到以机油为唯一碳源进行生长代谢的混合菌群。利用此混合菌群进行的降解实验结果表明,该菌群对高浓度机油废水具有较强的降解能力,初始含机油约2.0g/L的人工废水,接种量为0.1%(菌体湿重/培养液体积),经过7d的降解,机油可降至403mg/L,降解率达81.4%;对不同浓度机油废水的降解实验结果表明,在静态实验条件下,机油质量浓度在不高于1000mg/L(含1075mg/L),混合菌群在降解过程中能自行从降解产酸的不良环境中恢复,机油质量浓度在2000mg/L以上,初期产酸较多,pH下降幅度较大,在7d的周期内,废水pH无法恢复,说明在降解后期仍有大量有机酸积累而未被彻底降解;与葡萄糖共基质的降解实验结果表明,经过7d的降解,不超过150mg/L的葡萄糖与1000mg/L机油组成的共基质体系中,机油降解基本不受葡萄糖加入的影响,但可加强早期的降解速率。而葡萄糖高于150mg/L时,则会对混合菌群的除油率产生抑制,抑制程度随着葡萄糖浓度的提高而加大。 Mixed culture bacteria living on machine oil as the sole carbon source was prepared from soil samples from petroleum-contaminated sites by screening and acclimation. Starting with 2 000 mg/L of machine oil and with an inoculum density of 0.1%, the bacteria removed 81.4% of oil after 7 d of biodegradation at 32 ℃. When the initial machine oil concentration was under 1 000 mg/L, degradation resumed after pH recovered from the initial decline; when the initial machine oil concentration was above 2 000 mg/L, the pH was not fully recovered resulting in less effective degradation. Starting with an oil concentration of 1 000 mg/L, the initial degradation rate was enhanced by adding a small amount of glucose （〈150 mg/L） ; however, a higher dose of glucose （〉150 mg/L） inhibited oil degradation.

关 键 词： 混合菌群 机油 含油废水 生物降解

领　　域： [环境科学与工程]