作　　者： ; ; ;

机构地区： 暨南大学生命科学技术学院生物工程学系

出　　处： 《热带海洋学报》 2007年第3期43-47,共5页

摘　　要： 观察凤眼莲Eichhornia crassipes根粉末及丙酮提取物对东海原甲藻Prorocentrum donghaiense生长的影响,比较分析根系丙酮提取物中的化学成分以及不同成分抑藻效果,探讨凤眼莲根对藻类生长的抑制作用及其化学基础。结果显示,1.5g.L-1以上的凤眼莲根粉末可完全抑制东海原甲藻的生长。实际浓度0.019g.L-1的凤眼莲根丙酮提取物对东海原甲藻可产生50%的抑制率。N-苯基-2-萘胺浓度为1mg.L-1时,第6天对东海原甲藻的抑制率超过60%。浓度50μl.L-1时,亚油酸对东海原甲藻的抑制率超过80%。气相色谱-质谱联用(GC-MS)或高效液相色谱(HPLC)检测显示,凤眼莲根丙酮提取物中含有一定量的亚油酸和N-苯基-2-萘胺,同时还有大量的长链脂肪酸如十六酸、9-十六碳烯酸等。结果表明,凤眼莲根可显著抑制东海原甲藻的生长,N-苯基-2-萘胺、亚油酸可能是凤眼莲根抑藻的主要因子。 The effects of dry powder and acetone extract from Eichhornia crassipes roots on the growth of Prorocentrum donghaiense Lu were observed. The components in the acetone extract from E. crassipes roots were analyzed by GC-MS and HPLC methods, and their inhibitory actions were compared to elucidate the chemical basis of the inhibition. The results showed that the growth of P. donghaiense was stimulated when the dry powder from E. crassipes roots was lower than 1.0 g · L ^-1, and the growth was completely inhibited when the concentration was higher than 1.5 g · L ^-1. The extract with a concentration of 0. 019 g · L ^-1 could inhibit the growth of P. donghaiense by 50%. The inhibitory rate reached＇above 60% in the sixth day when N-phenyl-2-naphthylamine was 1 mg · L ^-1. The inhibitory rate reached above 82% when linoleic acid was 50 μl · L ^-1. The results of GC-MS or HPLC showed that in addition to N-phenyl- 2-naphthylamine and linoleic acid, long chain fatty acids such as hexadecanoic acid and 9-hexaaecenoic acid also existed in relatively high contents in the extract. These suggested that E. crassipes roots can remarkably inhibit P. donghaiense, and N-phenyl-2-naphthylamine and linoleic acid may play important roles in the inhibition.

关 键 词： 凤眼莲 东海原甲藻 苯基 萘胺 亚油酸 抑制作用

领　　域： [环境科学与工程]