作　　者： ; ; ; ;

机构地区： 华南理工大学轻工与食品学院

出　　处： 《华南理工大学学报（自然科学版）》 2007年第4期46-51,共6页

摘　　要： 为得到高纯度的凝胶多糖,采用分级膜(30 ku,100 ku)超滤同时耦合溶析结晶的方法——循环超滤-溶析结晶(CUSOC)法对凝胶多糖粗品进行了分级纯化,制备出了纯度分别达96.5%和93.3%的凝胶多糖产品,并对100 ku膜截留液所精制的多糖(CUD3)的组成进行了初步分析.结果表明,整个工艺过程的多糖截留率都在95%以上,多糖聚合物总收率约为93.10%.紫外光谱和纸层析鉴定表明,CUSOC方法能有效除去大部分小分子色素及核酸类杂质,凝胶多糖的水解物为单一葡萄糖.红外光谱分析表明,该多糖属β-D-糖苷键构型. In order to obtain curdlan with high purity, a new technology named Cyclic Uhrafihration-Solvating-Out Crystallization （CUSOC） was developed to separate and purify crude curdlan. This technology coupled a fractional uhrafiltration separation system as well as two portions （is 30 -100ku, more than 100ku） , with the solvating-out crystallization, by which curdlan with the purities of 96.5% and 93.3% , were obtained. The composition of CUD3 obtained with 100ku membrane was then preliminarily analyzed. The results show that, in the CUSOC process, the rejection rates are all up to 95% when using 30ku and 100ku membrane, and the total recovery of polysaccharides in the whole process reaches about 93.10%. It is also indicated by UV spectrum and paper chromatography that most pigment and nucleic acid impurity can be effectively removed in the process of CUSOC, and that the hydrolysis of CUD3 is a mono-ingredient only composed of glucose. Moreover, the IR spectrum reveals that CUD3 possess a β-D- glycoside linkage.

关 键 词： 凝胶多糖 分离 纯化 超滤 溶析结晶 过程耦合

领　　域： [轻工技术与工程] [轻工技术与工程] [生物学]