机构地区: 华南农业大学资源环境学院植物检疫线虫检测与防疫研究中心
出 处: 《中国生物防治》 2007年第2期151-155,共5页
摘 要: 液体培养的稻瘟病菌菌丝经过滤,减压浓缩,乙醇沉淀,透析,离心,获得滤液活性物质CFS。以CFS为激发子,研究其对玉米抗病性的诱导作用。结果表明,CFS能够诱导玉米对大斑病菌和弯孢叶斑病菌产生抗病性。CFS的浓度与诱抗效果呈显著正相关关系,在0.01到0.5μg/μl葡萄糖当量的浓度范围内,随着激发子浓度的增加,其诱导的抗病性增强;当CFS浓度高于0.5μg/μl,其诱导的抗病性不再增强。CFS的最高诱抗效果(50%左右)出现在处理后的2~3d,之后逐渐下降,到10d降至20%左右。 After filtration, decompression concentration, ethanol deposition, dialysis and centrifugation, cultural fluids filtrated substance (CFS) was obtained from hyphal of the rice blast fungus Magnaporthe grisea. Studies were carriedout on the resistance of corn induced by CFS as an elicitor. The results showed that CFS could increase resistance in corn against the fungal pathogens: Exserohilum turcicum (northern leaf blight) and Curvularia lunata (curvalaria leaf spot). There were significant positive corre- lation between the concentration of CFS and the efficiency of induced resistance. Ranging from 0.01 to 0.5 μg/μl sugar concentration, increasing CFS concentration raised the resistance effect, but exceeding down to 20 percents till 10 d.