作　　者： ; ; ; ;

机构地区： 四川农业大学动物科技学院水产养殖系

出　　处： 《中国水产科学》 2007年第3期457-465,共9页

摘　　要： 对2004-2005年四川发生的斑点叉尾(Ictalunes punctatus)急性流行性传染病进行病原学和病理学研究,从自然发病的斑点叉尾肝、肾分离到1株致病菌(CCF00024)。经人工感染实验证实该菌为斑点叉尾急性流行性传染病的病原菌。根据其形态和生理生化特性鉴定其为嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia)。病鱼的眼观病变主要表现为体表(特别是腹部和下颌)充血、出血和褪色斑,腹部膨大,腹腔内充有淡黄色或带血的腹水,胃肠道黏膜充血、出血,肠道发生套叠,甚至肠脱,肠腔内充满淡黄色或含血的黏液。组织学病变主要表现为全身组织器官广泛性水肿,出血、变性、坏死,以及中性白细胞、巨噬细胞等炎症细胞浸润,特别是骨骼肌、肝、脾、肾和胃肠道的损伤较为严重。细胞病理学表现为,病鱼肝、脾、肾肠道等器官的细胞均有较为严重的损伤,细胞肿胀,超微结构被破坏,特别是线粒体和细胞核的损伤明显。线粒体表现为肿胀,嵴断裂或溶解消失,呈空囊状;细胞核变形,染色质溶解或发生浓缩、裂解边移,甚至形成类似凋亡小体的结构。 In 2004 and 2005, an acute epidemic disease in channel catfish（ Ictalunes punctatus ） broke out in many farms of Sichuan Province. The pathogen and the pathology of diseased channel catfish were studied. A bacterial strain named CCF00024 was isolated from the kindey and liver of the channel catfish with the acute epidemic disease. The isolated bacterium was proved to be the pathogen of the disease by infection experiment. The pathogenic bacterium was identified as Stenotrophomonas rnaltophilia on the basis of their morphological features, physiological and biochemical characteristics. Examination on perceived changes showed congestion and hemorrhage in the body surface, especially in the abdomen and lower jaw, and many irregular, variably sized areas of depigmentation in the body surface, distension of abdomen, yellowish or flooded fluid in the peritoneal cavity, congestion and hemorrhage in mucosa of gastrointestinal tract and bloody mucus and fluid in the lumen, intussusception in rectum and even prolapse. Histopathological examination showed severe edema, hemorrhage, degeneration and necrotic changes in many organs including inflammatory cell infiltration; especially, serious lesions occurred in the kidney, liver, gastrointestinal tract, spleen and skeletal muscle. The kidney showed edema, degeneration and necrosis in renal tubular epithelia, and necrosis with macrophages and neutrophi infiltration in the hematopoietic tissue. The liver showed edema, vacuolar degeneration and necrosis. Epithelia mucosa of the gastrointestinal tract experienced degeneration and necrosis; the lamina propria and tunica submucosa showed edema and infiltration with lyrnphocytes,macrophages and neutrophi. The spleen showed congestion, hemorrhage, diminution in lymphoid tissue and infiltration with macrophages and neutrophi. Degeneration and necrosis with macrophages and neutrophi infiltration appeared in skeletal muscles. Cytopathological changes showed that swelling and ultrastructure broking in visceral organ cells. The mitoch

关 键 词： 斑点叉尾鲴 急性流行性传染病 嗜麦芽寡养单胞菌 病理学

领　　域： [农业科学] [农业科学]