作　　者： ; ; ; ; ; ; ;

机构地区： 中国农业大学生物学院农业生物技术国家重点实验室

出　　处： 《动物学报》 2007年第2期339-345,共7页

摘　　要： 本文通过新生大鼠原代培养获取骨骼肌肌管,利用水平回转器模拟失重效应,通过F-actin/G-actin以及F-actin/pERK免疫细胞化学染色,观察研究了模拟失重对肌管形态、微丝及磷酸化ERK表达的影响。通过建立的航天飞行失重性肌萎缩的细胞学模型研究发现:回转后肌管变细,F-actin染色减弱伴有G-actin染色增强,同时pERK染色减弱。表明回转模拟失重条件下肌管发生萎缩、微丝解聚并伴随信号转导活性分子磷酸化ERK表达下降。 Primary cultures of rat myotubes were clinorotated to simulate weightlessness, and subsequent effects of this procedure were observed using inverted phase contrast microscopy. Effects of weightlessness on aetin and aetivitation of extraeellular signalregulated kinase （ERK） were studied by laser confocal microscopy, after immunocytochemical staining for F-actin/G-actin and F/ aetin/pERK. Using established criteria for studies of the effects of spaceflight upon muscle atrophy, results showed that ciinoration caused myotubes to become thinner. Staining of F-actin and pERK decreased after clinoration, and staining of G-actin increased. Results indicated that disruption of the actin cytosketeton, and ERK activation was inhibited by cIonorotation. Thinning of the myotube is the structural and functional basis of muscle atrophy caused by weightlessness .

关 键 词： 模拟失重 回转器 肌管 微丝

领　　域： [生物学] [生物学]