作　　者： ; ; ; ;

机构地区： 西北农林科技大学食品科学与工程学院

出　　处： 《中国粮油学报》 2007年第2期79-82,共4页

摘　　要： 用气相色谱-质谱联用（GC-MS）方法对超，临界CO2萃取出的葵花籽油、南瓜籽油、山楂籽油和省沽油籽油中角鲨烯和维生素E进行分析。植物油的萃取采用超临界CO2萃取技术，然后用GC—MS方法分析，用内标标准曲线法同时测定四种植物油中角鲨烯和维生素E的含量。结果表明：所建立的定量分析方法平均回收率在96．4％～102．2％之间，相对标准偏差小于3．0％，线性相关系数大于0．999，方法检测限在2．0～2．5mg／L之间；四种植物油中角鲨烯和维生素E含量分别为（mg／g）：0．30和1．22（葵花籽油），1．98和0．50（南瓜籽油），2．92和2．31（山楂籽油），65．23和27．60（省沽油籽油）；其中省沽油籽油中角鲨烯和维生素E含量远远高于常用食用油。 A method for analysis of squalene and vitamin E in vegetable oils by gas chromatography - mass spectrometry （ GC - MS） method was developed. Four kinds of functional vegetable oil, sunflower seed oil, pumpkin seed oil, hawthorn seed oil, and Staphylea bumalda DC seed oil were extracted by supercritical CO2 fluid. Squalene and vitamin E in the oils were simultaneously determined by using GC -MS method with n -dotriacontane as the internal standard. The results show that the average added standard recovery of the developed method is in the range of 96.4 % - 102.2 % ; the relative standard deviations （RSD） are less than 3.0 % ; the correlation coefficients are more than 0. 999 and the detection limits are in the range of 2.0 - 2.5 mg/L. The contents （rag/g） of squalene and vitamin E in the vegetable oils are respectively 0.30 and 1.22 for sunflower seed oil, 1.98 and 0.50 for pumpkin seed oil, 2.92 and 2.31 for hawthorn seed oil, 65.23 and 27.60 for Staphylea bumalda DC seed oil. The contents of squalene and vitamin E in Staphylea bumalda DC seed oil are much higher than those in common edible oils.

关 键 词： 超 临界 萃取 气相色谱 质谱植物油 角鲨烯 维生素

领　　域： [轻工技术与工程] [轻工技术与工程]