作　　者： ; ; ; ; ; ;

机构地区： 华南农业大学兽医学院临床兽医系

出　　处： 《中国兽医科学》 2007年第4期342-345,共4页

摘　　要： 为进一步了解铜对生物体的生物学效应,选用硫酸铜作为铜源,向即时分离的肉鸡肝细胞线粒体培养液内加入终浓度分别为5、10、20μmol/L的铜(以铜计),孵育10 min后,观察肝细胞线粒体膜通透性及呼吸的变化。结果显示,铜对线粒体膜通透性转换孔有较显著的影响,造成线粒体不同程度的肿胀,表现为线粒体悬液在540 nm处的吸光值下降,且铜浓度越高,吸光值下降越明显,在孵育6 min后3种浓度的吸光值下降百分比差异显著(P<0.05)。随着铜浓度的升高,线粒体呼吸逐渐减弱,RCR、P/O、OPR均显著降低(P<0.05),这些变化可能是微量元素铜对生物机体产生毒害作用的本质原因之一。 To further understand the biologic functions of copper in animals, using copper（ Ⅱ ） sulfate anhydrous as the source of copper,mitochondrial permeability and respiration were observed after incubating the mitochondria which was freshly isolated from broiler hepatocyte with 5,10 and 20μmol/L of final concentration copper for 10 min, respectively. The effect of copper on the mitochondrial permeability transition pore was significant, resulting in swelling of mitochondria to some degree which was manifested by decrease of absorbance at 540 nm and the absorbance decreased dramatically with increase of copper concentration. After incubating for 6 min, the percentage of absorbance decrease among the above 3 concentrations was significant（P〈0.05）. Similarly, the respiration of mitochondria worn off with increase of copper concentration, and the values of RCR, P/O and OPR decreased significantly correspondingly （P〈0.05）. The results showed that these changes may represent one of the essential reasons of copper poison in organisms.

关 键 词： 铜 线粒体 线粒体膜通透性转换孔 呼吸

领　　域： [农业科学] [农业科学] [农业科学]