作　　者： ; ; ; ;

机构地区： 华南农业大学

出　　处： 《华中农业大学学报》 2007年第2期147-150,共4页

摘　　要： 利用PLACE数据库,对大麦β-1,3-葡聚糖酶同功酶基因PGⅢ启动子的可能顺式作用元件进行分析,并设计特异性引物。通过5′-缺失法获得了不同大小的启动子片段,分别与报告基因gus(β-葡聚糖酸醛苷酶基因)耦联,构建植物表达载体,通过农杆菌介导法转化水稻胚性愈伤组织。GUS组织化学染色结果表明,稻瘟菌来源的激发子可以诱导PGⅢ启动子不同缺失体驱动的gus表达。荧光法结果表明,缺失体P1(-1 107 bp)具有最高的激发子诱导活性,而缺失体P4(-359 bp)驱动的gus表达量迅速降低,缺失体P5的诱导活性最低。这表明转录起始位点上游-1 107 bp序列是保持启动子最高活性所必需的,而-572--359 bp的序列是保持激发子诱导活性所必需的。GCC框和GTAC序列可能是激发子诱导表达的顺式作用元件。 To define the cis-elements in the elevated expression of Pore by the elicitor, a series of 5＇ deletion mutants of PGⅢ were obtained by PLACE analyses. Different 5＇-deletion fragments were fused to gus gene, and introduced into rice via A. turnefaciens. Histochemical staining showed that GUS activity was detected in transformed rice calli containing each deletion mutant after elicitor-treatment. The value of GUS activity established by the P1-P3 deletion mutants were significantly increased 19-fold to 47- folds respectively after elicitor treatment versus water treatment by spectrofluorophotometric analysis. The GUS activity regulated by P1 was higher than other deletion mutants after elicitor treatment. The value of GUS activity established by the P4 deletion mutants were significantly decreased. The results suggested that the sequences between -572 bp and -359 bp were required for high level activation by elicitor. And -1 107 bp upstream of the transcription start site Was required for maximal levels of elicitor-inducibility.

关 键 词： 水稻 启动子 激发子 活性

领　　域： [农业科学] [农业科学]