作　　者： ; ; ; ; ; ; ;

机构地区： 深圳市血液中心

出　　处： 《现代检验医学杂志》 2007年第2期49-51,共3页

摘　　要： 目的比较全自动工作站与两种人工法抽提全血基因组DNA的质和量,总结三种方法的特点及提供一些建议。方法三种方法均选取同样36份样本抽提基因组DNA,并比较其浓度和纯度,并计算三种方法所需的费用。结果三种方法提取的基因组DNA均能达到相关分子生物学实验的要求,但在操作程序、价格和全血需要量等方面都存在较大差异。结论全自动工作站用全血量少,价格比较便宜,适合大批量全血样本抽提基因组DNA。Gentra法和胍盐酸法均适用于新鲜全血和冻融过的全血,胍盐酸法还适用于有血凝块的全血。 Objective To compare the quality and quantity from whole blood samples for genomic DNA extraction by the workstation and two manual methods. Methods Three methods were used to extract genomic DNA from same thirty-six samples ,and the results were compared in quantity ,quality and the cost. Results The three methods all could give high field of DNA,but there were very different in operation procedure and the cost. Conclusion Workstation is fit for extration genomic DNA from lots of samples because of a little blood and low cost. The Gentra method and the hydrochloric acid carbamidine method are fit for fresh and frozen whole blood, and the hydrochloric acid carbamidine method can be used to extract genomic DNA from blood clots.

关 键 词： 全自动 基因组 全血

领　　域： [生物学]