作　　者： ; ; ; ; ;

机构地区： 广西农业科学院

出　　处： 《广西师范大学学报（自然科学版）》 2007年第1期86-90,共5页

摘　　要： 通过PCR从苋科植物野苋菜Amaranthus viridis L.基因组DNA中扩增出野苋菜凝集素的核基因片段AVA。序列分析结果表明该基因为1831 bp,含有一个922 bp的内含子和两个分别为212 bp和697 bp的外显子。采用反向PCR技术获得了该基因的编码区克隆。分别构建含有内含子和不含内含子的AVA基因植物表达载体pBI121AVA-GUS和pBI121AVAc-GUS,通过根癌农杆菌介导法转化烟草。PCR、GUS检测结果均表明AVA基因不仅已整合到烟草基因组DNA中,而且初步表明转基因烟草有AVA蛋白表达。抗蚜实验表明含内含子和无内含子的AVA基因在转基因烟草中的抗蚜能力不同,转AVA和AVAc烟草对蚜虫的抑制率分别为60.81%和50.63%,有的植株高达97%。实验结果表明所克隆的AVA基因是具有抗蚜能力的苋菜凝集素基因家族中的新成员。 The Amaranthus viridis agglutinin （AVA） gene from Amaranthus viridis L. was cloned by PCR. The gene is 1 831 bp long ,include one 922 bp intron and two exons of 212 bp and 697 bp. The AVA gene with and without intron were inserted downstream of 35S promoter into a vetor pBI121 to get pBI121AVA-GUS and pBI121AVAc-GUS vetor respectively. After transformed into tobacco （Nicotinana tobatum） plant with leaf disc transformation. Results of PCR and GUS detection showed that the trasformants were transgenic tobacco plants. The results from insect bioassay with peach aphid （Myzus persicae） showed that the transgenic plants of pBI121AVA-GUS and pBI121AVAc-GUS were aphid resistant,evidenced by a 60. 81%-50. 63% reduction in insect population density,some plants were more than 97 %. AVA gene of aphid resistance is regarded as a new member in Amaranthus lectin families.

关 键 词： 植物学 野苋菜 基因 转基因烟草 抗蚜性

领　　域： [生物学]